Establishment of poly(ADP-ribose) polymerase-deficient mouse embryonic stem cell lines
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概要
- 論文の詳細を見る
Many studies revealed that poly(ADP-ribose) polymerase (Pare) is involved in DNA-damage recovery, cell-death induction and maintenance of genomic stability. We generated mouse Parp+/- embryonic stem (ES) cell lines by disrupting one allele of Parp exon 1 with a neomycin-resistance gene-cassette and subsequently produced Parp-/- ES cells by disrupting the remaining allele with a puromycin-resistance genecassette. Parp activity was decreased to half in Parp+/- ES cell clones and lost in Parp-/- ES cell clones. Growth rates of Parp+/- and Parp-/- ES cell clones were similar to that of parental J1 ES cells, indicating that Parp is not required for ES cell proliferation. These Parp-/- ES cells will be useful to study biological role of poly(ADP-ribosyl)ation reaction.
- 日本学士院の論文
著者
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WAKABAYASHI Keiji
Cancer Prevention Division, National Cancer Center Research Institute
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NAKAGAMA Hitoshi
Biochemistry Division, National Cancer Center Research Institute
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Nakagama Hitoshi
Biochemistry Division National Cancer Center Research Institute
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Mori Masahiko
Department Of Oral Pathology Osaka Dental University
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SUGIMURA Takashi
Biochemistry Division, National Cancer Center Research Institute
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NOZAKI Tadashige
Biochemistry Division, National Cancer Center Research Institute
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SUZUKI Hiroshi
Chugai Pharmaceutical Co., Ltd.
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MASUTANI Mitsuko
Biochemistry Division, National Cancer Center Research Institute
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Wakabayashi Keiji
Cancer Prevention Basic Research Project National Cancer Center Research Institute
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Nozaki T
National Cancer Center Res. Inst. Tokyo Jpn
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Mori M
Department Of Oral And Maxillofacial Surgery School Of Dentistry Asahi University
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Mori M
Department Of Oral Surgery Gifu College Of Dentistry
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OCHIYA Takahiro
Section for Studies on Metastasis, National Cancer Center Research Institute
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Muto Masahiro
Radiat. Hazards Res. G. Nirs
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Masutani Mitsuko
Biochemistry Division National Cancer Center Research Institute
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Mori Masahiko
Radiation Hazards Research Group National Institute Of Radiological Sciences
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Noda T
National Cancer Center Hospital Tokyo
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Masutani M
National Cancer Cent. Res. Inst. Tokyo Jpn
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Muto Masahiro
Department Of Oral Pathology Osaka Dental University
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NISHIYAMA Eiko
Biochemistry Division, National Cancer Center Research Institute
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Ochiya Takahiro
Section For Studies On Metastasis National Cancer Center Research Institute
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Sugimura Takashi
Biochemistry Division National Cancer Center
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Mori Masahiko
Div. Radiobiology And Biodosimetory
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OCHIYA Takahiro
Section for Studies of Metastasis, National Cancer Center Research Institute
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