Changes in Glycosaminoglycan Characteristics during Progression of a Human Gingival Carcinoma Xenograft Line in Nude Mice
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概要
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We investigated changes in the glycosaminoglycans (GAGs) during progression of a human gingival carcinoma xenograft line, GK-1, in nude mice. The GAGs extracted from cancers 3, 5, 7, 10 and 15 weeks after transplantation consisted of hyaluronic acid (HA), chondroitin sulfate (CS) and heparan sulfate (HS) as major components, and dermatan sulfate (DS) as a trace component for all cancers. HPLC analysis revealed that the HA content per defatted tissue dry weight increased in the cancers 5 weeks after transplantation compared to those of 3 weeks (p<0.05), while CS for cancers at 10 weeks decreased compared with 7 weeks (p%lt;0.05). However, HS showed no significant change. Both the CS and DS contained primarily 4-sulfated disaccharide units. Immunohistochemical staining with antibody 2-B-6 for the PGs having ΔDi-4S produced by chondroitinase ABC digestion showed that CS is located in the tissue surrouding the cancer nests and mass. These results indicate that the location of accumulation of CS, which primarily contains 4-sulfated disaccharide units, plays an important role in cancer progression.
- 大阪歯科学会の論文
著者
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Kamada Aiko
Department of Biochemistry Osaka Dental University
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Ida Masayasu
Department Of Biochemistry Osaka Dental University
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