Stability of Enzyme Preparations on Radiosterilization

概要

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The sterilizing effects of gamma rays and the stabilities of enzymes to gamma radiation were studied. The sterilizing effects of gamma rays were similar in powder and liquid states of four enzymes with E. coli and Staph. aureus as contaminants. D_<10> values for E. coli solution and E. coli lactose powder were both 37 krad, and those for Staph. aureus solution and Staph. aureus lactose powder were 30 krad and 25 krad, respectively. The stability of enzymatic activity to gamma radiation depended on the form of the enzyme material. In solution the activity of each enzyme was reduced by a dose of 10 krad. Trypsin and kallikrein were inactivated completely by 50 krad and chymotrypsin and bromelain by 100 krad. However, in the powder state, no enzyme was found to show loss of activity at less than 500 krad. All the enzymes retained more than 90% activity after 1 Mrad irradiation, and substantial activities were retained after 5 Mrad. No difference in the stability of enzymatic activity was found between the powder state and lactose mixture. When water or ethanol was present in the lactose mixture, the stability to gamma radiation was greatly reduced.
公益社団法人日本薬学会の論文
1980-04-25