Polynucleotides. LIX. Synthesis and Properties of a Deoxyribohexanucleotide corresponding to the EcoRI Recognition Sequence
スポンサーリンク
概要
- 論文の詳細を見る
A deoxyribohexanucleotide corresponding to a restriction endonuclease (Eco RI) recognition sequence, d-G-A-A-T-T-C, was synthesized by stepwise condensation of mononucleotides using dicyclohexylcarbodiimide (DCC) or 2,4,6-triisopropylbenzenesulfonyl chloride (TPS) as the condensing reagent. Protected oligonucleotides were separated by ion-exchange chromatography or gel filtration and the deblocked hexanucleotide was purified by DEAE-cellulose column chromatography in the presence of 7 M urea. The product was characterized by enzymatic hydrolysis and its temperature-absorption profile was measured at high ionic strength.
- 1980-01-25
著者
-
大塚 栄子
Faculty Of Pharmaceutical Sciences Hokkaido University
-
池原 森男
Faculty Of Pharmaceutical Sciences Osaka University
-
福元 良一
Faculty of Pharmaceutical Sciences, Osaka University
関連論文
- Activity of Artificial Mutant Variants of Human Growth Hormone Changes in Charged Residues around 62-67
- Activity of Artificial Mutant Variants of Human Growth Hormone Deficient in a Disulfide Bond between Cys53 and Cys165
- 高等植物FEN-1遺伝子のクローニングおよびその解析
- Synthesis of 8,5'-Anhydro-8-mercaptoadenosine
- Studies on tRNA and Related Compounds. XXXVII. Synthesis and Physical Properties of 2'- or 3'-O-(o-Nitrobenzyl) nucleosides : the Use of o-Nitrophenyldiazomethane as a Synthetic Reagent
- Studies on Transfer Ribonucleic Acids and Related Compounds. XVI. Synthesis of Ribooligonucleotides using a Photosensitive o-Nitrobenzyl Protection for the 2'-Hydroxyl Group
- Molecular Dynamics and Free Energy Perturbation Calculations on the Mutation of Tyrosine 45 to Tryptophan in Ribonuclease T_1
- Synthesis and Expression of a Gene for Human Tumor Necrosis Factor
- Synthesis of a Gene Coding for Human Lysozyme
- Synthesis and Properties of d(ATACGCGTAT) and Its Derivatives Containing One and Two 5-Methylcytosine Residues. : Effect of the Methylation on Deoxyribonucleic Acid Conformation
- Polynucleotides. LXIII. Solution Conformation of Oligodeoxyribonucleotides containing an Alternating dC-dG Sequence which can form a Left-handed Double Helix
- Studies on Nucleosides and Nucleotides. LXXXVIII. : Purine Cyclonucleosides. XLIII. : ^C NMR Spectra of 2'-Substituted 2'-Deoxyadenosines. Substituent Effects on the Chemical Shifts in the Furanose Ring System
- Polynucleotides. LXI. Synthesis and Properties of Dinucleoside Monophosphates Containing 8,2'-S-Cycloadenosine and 8,2'-S-Cycloinosine Residues. Sequence Dependency of the Stability of the Stacking Conformation
- 2P014紫外線損傷DNA切断抗体の創製
- c-Mycとp21^蛋白質間での直接結合による相互の不活化
- アンチセンス核酸によるリボザイムの切断活性の誘導
- Dewar isomer認識一本鎖抗体の構築
- 大腸菌における抗(6-4)光産物一本鎖抗体の発現と生物学的効果
- 真核生物の複製フォーク複合体の分子集合
- NMRによる抗チミンダイマー抗体のDNA認識機構の解析
- ランダム変異導入による抗体の作製
- 紫外線損傷DNAを認識する抗体の抗原結合部位のNMR解析
- (6-4)光産物を特異的に認識する可溶化型一本鎖抗体の作製
- ヒトPCNAとFEN-1の相互作用の解析
- ヒトPCNAの三量体構造と機能の解析
- ヒトPCNAとp21^の相互作用解析
- ヒト PCNA の構造と機能
- リボザイムの遺伝子治療への応用 (アンチセンス医薬品の研究開発)
- Purification and Characterization of the Product of Chemically Synthesized Human Growth Hormone Gene Expression in Escherichia coli(Biological,Chemical)
- A Large-Scale Synthesis of a Nonadecadeoxyribonucleotide Duplex Having a Sequence Identical to That of Phage φ80 O_R2 by the Phosphoro-p-anisidate Method
- Studies on Transfer Ribonucleic Acids (tRNA) and Related Compounds. XLIX. Solid-Phase Synthesis of Nonadecaribonucleotide Corresponding to the 5'-Half of the Formylmethionine tRNA Deficient in D-Loop and Stem Region
- PARTIAL PURIFICATION AND BIOLOGICAL ACTIVITY OF THE PRODUCT OF CHEMICALLY SYNTHESIZED HUMAN GROWTH HORMONE GENE EXPRESSION IN ESCHERICHIA COLI
- Deoxyribonucleic Acids (DNA) and Related Compounds. XIII. Synthesis of DNA Duplexes Containing a Ribosome Binding Site
- Deoxyribonucleic Acids and Related Compounds. XII. Polymer Support Synthesis of a 46-mer Duplex Containing the Promoter of Galactose Operon of Escherichia coli
- Deoxyribonucleic Acids and Related Compounds. VIII. Solid-Phase Synthesis of Deoxyribooligonucleotides with 3'-Modification by Elongation in the 3'-Direction
- Studies on Transfer Ribonucleic Acids and Related Compounds. XLIV. A Large-Scale Synthesis of the Anticodon Heptanucleotide of Formyl-methionine Transfer Ribonucleic Acid by Using 2'-O-Tetrahydrofuranylnucleosides
- Studies on Transfer Ribonucleic Acids and Related Compounds. XLIII. Synthesis of Oligoribonucleotides by using 5'-Selective Phosphorylation of 2'-O-Tetrahydrofuranyl Nucleosides
- Studies on Transfer Ribonucleic Acids and Related Compounds. XLI. Synthesis of tRNA Fragments containing Modified Nucleosides
- Studies on Deoxynucleic Acids and Related Compounds. VI. Synthesis of Oligodeoxyribonucleotides containing Recognition Sequences for Restriction Endonucleases
- Studies on Deoxynucleic Acids and Related Compounds. IV. Syntheses of an Octanucleotide containing a Recognition Site for Restriction Enzyme Eco RI and of an Arabinosyladenine Analog
- A NEW SYNTHETIC METHOD FOR 5'-TRIPHOSPHORYL-ADENYLYL-(2'-5')-ADENYLYL-(2'-5')-ADENOSINE (2-5A) AND 2-5A CORE USING 3'-O-TETRAHYDROFURANYL-ADENOSINE DERIVATIVES
- Studies on Deoxyribonucleic Acids and Related Compounds. III. Synthesis of Oligodeoxyribonucleotides Complementary to the Anticodon Loop of Escherichia coli tRNA^_f by an Improved Triester Method
- Studies on Deoxyribonucleic Acids and Related Compounds. II. Synthesis of a Decanucleotide containing a Restriction Enzyme (PstI) Recognition Site
- Studies on Transfer Ribonucleic Acids and Related Compounds. XXXIV. Stepwise Diester or Partial Triester Synthesis of Penta-to Octanucleotides Corresponding to Residues 41-46,47-54,61-65 and 66-71 of tRNA^_f of E. coli
- Studies on Transfer Ribonucleic Acids and Related Compounds. XXV. Synthesis of E. coli tRNA^_f 5'-Terminal Tetranucleotide C-G-C-Gp and Its Sequence Analog U-G-C-Gp
- Studies on Transfer Ribonucleic Acids and Related Compounds. XVII. Studies on Protecting Groups of Trisubstituted Phosphates in the Synthesis of Ribooligonucleotides
- In Vitro Replication Study of Modified Bases in ras Sequences
- Blockwise Mechanical Synthesis of Oligonucleotides by the Phosphoramidite Method(Organic,Chemical)
- Deoxyribonucleic Acids and Related Compounds. XXII. : Synthesis of Genes for Human Nerve Growth Factor and Its Fused Protein
- A NEW SOLID-PHASE SYNTHESIS OF RIBOOLIGONUCLEOTIDES USING THE 3'-PHOSPHORO-p-ANISIDATE PROTECTING GROUP FOR STEPWISE SYNTHESIS IN THE 3'-DIRECTION
- 遺伝子合成と生物学の革命(挑戦者からのメッセージ)
- 奨励賞受賞 紙谷浩之氏の業績
- 核酸合成と分子認識
- 研究費のバブル時代とならないために
- 核酸の合成と機能に関する研究.合成ras遺伝子関連の研究を中心として
- DNA/RNA合成機 (バイオ高性能機器導入・共同利用マニュアル) -- (核酸関連機器)
- リボザイム
- ハンマ-ヘッド型リボザイムの触媒活性に必要なMg2+の結合部位 (1991年の化学-10-)
- RNAを配列特異的に切断するリボザイム
- Synthesis of Wobble Pairing Oligoribonucleotides
- Synthesis and Thermal Stability of Dodecadeoxyribonucleotides Containing Deoxyinosine Pairing with Four Major Bases
- 蛋白質工学とRNA工学
- Synthesis and Properties of Hexanucleotides Containing Deoxyinosine
- Preparation and Characterization of Monoclonal Antibodies Specific for Ribonuclese Inhibitor from Rat Liver
- Overproduction of Cellular and Activated Ha-ras Proteins by Mutating a Synthetic Gene(Biological,Chemical)
- Polynucleotides. XXXIV. Ultraviolet Absorption and Circular Dichroism of ApUpG Analogs containing Modified Adenosine Residues
- Studies of Nucleosides and Nucleotides. LVI. A Versatile Method for the Synthesis of 8-Mercaptoadenosine Nucleotides
- ショウジョウバエの(6-4)photoproductに特異的に結合し切断するヌクレアーゼについて
- Studies on Transfer Ribonucleic Acids and Related Compounds. VII. Synthesis and Properties of Cyclic Oligoadenylic Acids
- リボザイムの構造と分子設計(アンチセンスとリボザイム) (核酸化学の新展開--新しい機能性分子を求めて) -- (核酸の機能)
- Ribozyme--酵素としてのRNA (1987年の化学-5-)
- ras遺伝子産物の活性と構造 (1986年の化学-4-)
- 遺伝子の人工合成とその発現 (1985年の化学)
- Synthesis of Guanosine 5'-Diphosphate.
- Synthesis of Guanosine Diphosphate Mannose
- ピリミジン2量体の変異作用
- Inhibition of c-Ha-ras Gene Expression by Hammerhead Ribozymes Containing a Stable C(UUCG)G Hairpin Loop
- Studies on Transfer Ribonucleic Acids and Related Compounds. XXXIX. Chemical Synthesis of Heptadeca- and Hexadecaribonucleotides corresponding to the 3'-Terminus of the tRNA^_f of E. coli
- Studies on Transfer Ribonucleic Acids and Related Compounds. XXXI. Synthesis of the 3', 5'-Bisphosphorylated Hexanucleotide corresponding to Bases (72-77) of tRNA^ from E. coli and Its Base Transition Analog
- Studies on Transfer Ribonucleic Acids and Related Compounds. XXI. : Synthesis and Properties of Guanine Rich Fragments from E. coli tRNA^_f 5'-End
- Studies on Transfer Ribonucleic Acid and Related Compounds. XIII. Synthesis of Trinucleoside Diphosphates, A-U-A and εA-U-A via a Triester Intermediate
- Studies of Nucleosides and Nucleotides. XXII. Phosphorylation of Adenosine using Borate Complex as Protecting Group for 2'-and 3'-Hydroxyl Groups.
- Studies on Coenzyme Analogs. XVII. A New Phosphorylating Agent, 2,6-Lupetidylphos-phorodichloridate, and Some Investigations on the Selective Phosphorylation.
- Studies on Transfer Ribonucleic Acids and Related Compounds. XXXVIII. A Rapid Method for the Synthesis of Ribooligonucleotides by using 3', 5'-Unsubstituted Nucleosides. Synthesis of a Hexanucleotide containing Anticodon Triplet of E. coli tRNA^_f.
- Studies on Transfer Ribonucleic Acids and Related Compounds. X. Synthesis of the Yeast Tyrosine tRNA 5'-Terminal Oligonucleotides
- ハンマ-ヘッド型およびヘアピン型リボザイムの細胞内活性 (1990年の化学-8-)
- RNA用制限酵素としてのリボザイムの設計 (1989年の化学-8-)
- RNA自己切断に必要な塩基配列 (1988年の化学-5-)
- Polynucleotides. XXIX. Synthesis of Deoxyribooligonucleotide Blocks by an Extraction Method
- Studies on Transfer Ribonucleic Acids and Related Compounds. XXVII. Linear and Cyclic Oligonucleotides obtained by Polymerization of Protected Ribonucleoside 3'-Phosphates
- RNA工学シリ-ズ-2-RNAの化学合成とリボザイム活性
- 遺伝情報伝達における損傷DNAの影響
- RNA酵素の設計と合成-2-RNA酵素のin vivo活性
- Selective Removal of Protecting Groups for Phosphomonoesters of Nucleotides by Anodic Oxidation
- Polynucleotides. XXVI. Synthesis of an AUG Analog, 8,2-Anhydro-8-oxy-9-β-D-arabinofuranosyladenine Phosphoryl-(3'-5')-uridylyl-(3'-5')-guanosine
- Polynucleotides. XX. Synthesis of an AUG Analog containing 8,5'-O-Cycloadenosine
- Interactions between Synthetic Nucleotide Analogs and Snake Venom 5'-Nucleotidase
- Studies on Coenzyme Analogs. IX. Synthesis of 6-Alkylamino-9-β-D-ribofuranosylpurine 5'-Monophosphate.
- Polynucleotides. LIX. Synthesis and Properties of a Deoxyribohexanucleotide corresponding to the EcoRI Recognition Sequence
- Studies on Transfer Ribonucleic Acid and Related Compounds. XI. A Comparative Study on Condensing Reagents in Syntheses of Ribooligonucleotides