Phospholipase Cによる家兎腹水多形核白血球からのLysosome酵素の遊離に対する抗炎症薬の作用

概要

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The polymorphonuclear (PMN) leucocyte suspension in the medium with or without Ca^<2+> was incubated with phospholipase C (PLC). In the medium without Ca^<2+>, PLC (0.001 U/ml) caused the release of lactate dehydrogenase (LDH) and lysosomal enzymes (aryl sulfatase and β-glucuronidase) from PMN leucocytes. The release of lysosomal enzymes was significantly inhibited by dexamethasone (0.01-1 μM), prednisolone (0.01-10 μM), and hydrocortisone (0.1-100 μM), but not by indomethacin (100 μM) and phenylbutazone (100 μM). In the medium with Ca^<2+>, PLC (1 U/ml) induced the secretion of lysosomal enzymes from PMN leucocytes without the release of LDH and loss of viability of leucocytes. This secretion of lysosomal enzymes was enhanced by the addition of 3 mM cysteine and significantly inhibited by the above anti-inflammatory drugs and colchicine in concentrations less than 10 μM. These methods may be useful as a simple test to check the effect of anti-inflammatory agents on the secretion of lysosomal enzymes.
公益社団法人日本薬学会の論文
1977-04-25