Purification, Characterization, and Gene Analysis of Catechol 2,3-Dioxygenase from the Aniline-Assimilating Bacterium Pseudomonas Species AW-2

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概要

• 論文の詳細を見る

• Catechol 2,3-dioxygenase (C23D) ; EC 1.13.1.2) was purified to homogeneity from a cell extract of Pseudomonas sp.A W-2 grown on aniline, and the purified C23D was characterized. The molecular mass estimated by gel filtration was 110 kDa. The enzyme dissociated into four identical subunits each with the molecular mass of 33 kDa. The enzyme had high activity for 3-methylcatechol as well as catechol, and differed from the enzyme from Pseudomonas putida mt-2,which carries the TOL plasmid, in optimal pH for catechol, extradiol cleavage activities for 3-methylcatechol and 4-methylcatechol, and immunochemical properties. The amino acid sequence deduced from a C23D gene, alnE, from Pseudomonas sp. AW-2 was 85.7% identical to that of 3-methylcatechol 2,3-dioxygenase from toluidine-assimilating Pseudomonas putida UCC22. AlnE was 44.1% identical to the C23D encoded by xylE in P. putida mt-2. Because XylE has low activity for 3-methylcatechol, these results suggest that the differences in substrate specificity for 3-methylcatechol among the C23Ds reflected their sequence similarity.

• 社団法人日本農芸化学会の論文
• 1998-04-23

著者

• Aoki K

  Department Of Applied Biological Chemistry Graduate School Of Agricultural Science Kobe University

• Takenaka Shinji

  Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University

• MURAKAMI Shuichiro

  Department of Bioscience, Biotechnology, and Agrochemistry, Faculty of Agriculture, Meiji University

• AOKI Kenji

  Department of Nutritional Management, Faculty of Nutritional Science, Sagami Women’s University

• Nakanishi Yoko

  Division Of Science Of Biological Resources Graduate School Of Science And Technology Kobe Universit

• SHINKE Ryu

  Department of Biofunctional Chemistry, Kobe University

• Nakanishi Y

  Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University

• KODAMA Noriko

  Division of Science of Biological Resources, Graduate School of Science and Technology, Kobe Univers

• TAKENAKA Shinji

  Division of Science of Biological Resources, Graduate School of Science and Technology, Kobe Univers

• Shinke R

  Laboratory Of Bio-functional Chemistry Division Of Bioscience The Graduate School Of Science And Tec

• Shinke Ryu

  Division Of Science Of Biological Resources The Graduate School Of Science And Technology

• Shinke Ryu

  Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University

• Kodama Noriko

  Division Of Science Of Biological Resources Graduate School Of Science And Technology Kobe Universit

• Murakami Shuichiro

  Dep. Of Bioscience Biotechnology And Agrochemistry Fac. Of Agriculture Meiji Univ.

• Aoki Kenji

  Department Of Nutritional Management Faculty Of Nutritional Science Sagami Women's University

• Takenaka Shinji

  Department Of Agrobioscience Graduate School Of Agricultural Science Kobe University

• Takenaka Shinji

  Division Of Science Of Biological Resources The Graduate School Of Science And Technology:kobe Unive

• Shinke Ryu

  Laboratory Of Bio-functional Chemistry Division Of Bioscience The Graduate School Of Science And Tec

• Murakami Shuichiro

  Department Of Bioscience Biotechnology And Agrochemistry Faculty Of Agriculture Meiji University

• Aoki Kenji

  Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University

• MURAKAMI Shuichiro

  Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University

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