Regulation by Two CatR Proteins that Differ in Binding Affinity to catB Promoters Expressing Two cat Gene Clusters(Biochemistry & Molecular Biology)

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概要

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• We isolated the two LysR-type regulatory proteins CatR_1 and CatR_2, which regulate the expression of cat_1 and cat_2 gene clusters, respectively, required for catechol degradation in the bacterium Frateuria sp. ANA-18. In a gel mobility shift assay using CatR_1 and the DNA fragment containing the catB_1 promoter region, the formation of two complexes, complex 1-1 (Cl-1) and complex 1-2 (Cl-2), was observed in the presence of cis, cis-muconate. On the other hand, CatR_2 and the DNA fragment containing the catB_2 promoter region formed only complex 2-2 (C2-2) at a lower concentration of cis, cis-muconate than that at which C1-1 and C1-2 were formed. As the concentration of cis, cis-muconate decreased, the production of the muconate cycloisomerase isozyme MC II encoded by catB_2 decreased as well as that of MC I encoded by catB_1. However, the amount of MC II synthesized was larger than that of MC I at low concentrations. On the basis of these results, we concluded that the catB_2 promoter was activated at low concentrations of cis, cis-muconate.

• 社団法人日本農芸化学会の論文
• 2001-10-23

著者

• Takenaka Shinji

  Laboratory of Applied Microbiology, Department of Biofunctional Chemistry, Faculty of Agriculture, K

• Murakami Shuichiro

  Laboratory of Applied Microbiology, Department of Biofunctional Chemistry, Faculty of Agriculture, K

• Aoki Kenji

  Laboratory of Applied Microbiology, Department of Biofunctional Chemistry, Faculty of Agriculture, K

• Aoki K

  Department Of Applied Biological Chemistry Graduate School Of Agricultural Science Kobe University

• Takenaka Shinji

  Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University

• Murakami S

  Kikkoman Corp. Chiba Pref. Jpn

• TAKASHIMA Atsushi

  Division of Bioscience, The Graduate School of Science and Technology, Kobe University

• Murakami Shuichiro

  Dep. Of Bioscience Biotechnology And Agrochemistry Fac. Of Agriculture Meiji Univ.

• Takashima Atsushi

  Division Of Bioscience The Graduate School Of Science And Technology Kobe University

• Takenaka Shinji

  Department Of Agrobioscience Graduate School Of Agricultural Science Kobe University

• Aoki K

  Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University

• Aoki Kenji

  Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University

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