Production of Catechol by Transpositional Mutants of Aniline-Assimilating Pseudomonas Species AW-2

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概要

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• Twenty-four transpositional mutants of aniline-assimilating Pseudomonas sp.AW-2 produced catechol from aniline and accumulated it in a cultural medium. Among the 24 mutants, strain B-9 produced a maximal amount of catechol (0.97 mg/ml) from 1 mg/ml of aniline in a 13.5-h growing culture containing pyruvate and casamino acids. The conversion rate of aniline to catechol was 82% on a molar basis. The catechol production by the resting cells increased in the presence of glycerol and citrate, and a maximal amount of catechol produced from 1 mg/ml of aniline reached 1.1 mg/ml at the conversion rate of 93% after 2 h of incubation. Enzymes catalyzing the synthesis of catechol from aniline were induced by aniline in strain B-9 cells.

• 社団法人日本生物工学会の論文
• 1996-11-25

著者

• Murakami Shuichiro

  Laboratory of Applied Microbiology, Department of Biofunctional Chemistry, Faculty of Agriculture, K

• Aoki Kenji

  Laboratory of Applied Microbiology, Department of Biofunctional Chemistry, Faculty of Agriculture, K

• Aoki K

  Department Of Applied Biological Chemistry Graduate School Of Agricultural Science Kobe University

• KODAMA Noriko

  Division of Science of Biological Resources, Graduate School of Science and Technology, Kobe Univers

• Shinke R

  Laboratory Of Bio-functional Chemistry Division Of Bioscience The Graduate School Of Science And Tec

• Shinke Ryu

  Division Of Science Of Biological Resources The Graduate School Of Science And Technology

• Kodama Noriko

  Division Of Science Of Biological Resources Graduate School Of Science And Technology Kobe Universit

• SHINKE RYU

  Laboratory of Applied Microbiology, Department of Biofunctional Chemistry, Faculty of Agriculture

• Murakami Shuichiro

  Dep. Of Bioscience Biotechnology And Agrochemistry Fac. Of Agriculture Meiji Univ.

• Shinke Ryu

  Laboratory Of Bio-functional Chemistry Division Of Bioscience The Graduate School Of Science And Tec

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