Cloning and Functional Analysis of Aniline Dioxygenase Gene Cluster, from Frateuria Species ANA-18, That Metabolizes Aniline via an ortho-Cleavage Pathway of Catechol(Microbiology & Fermentation Technology)

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概要

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• Genes encoding an aniline dioxygenase of Frateuria sp. ANA-18, which metabolizes aniline via the ortho-cleavage pathway of catechol, were cloned and named tdn genes. The tdn genes were located on the chromosomal DNA of this bacterium and weren't clustered with catechol-degrading gene clusters. These results show that the ANA-18 aniline-degrading gene cluster is constructionally different from Pseudomonas tdn and Acinetobacter atd gene clusters, which degrade aniline via the meta-cleavage pathway of catechol and organize catechol-metabolic genes in the gene clusters. When cloned tdnQTA1A2B genes were expressed in Eschherichia coli, aniline dioxygenase activity was observed. Southern blot analysis revealed that homologues of the tdnA1A2B genes didn't exist in strain ANA-18. Disruption of the tdnA1A2 genes gave the parent strain ANA-18 a defect in aniline metabolism. On the basis of these results, we concluded that only the cloned tdn genes function as genes encoding aniline dioxygenase in strain ANA-18 although this bacterium had two catechol-degrading gene clusters.

• 社団法人日本農芸化学会の論文
• 2003-11-23

著者

• Takenaka Shinji

  Laboratory of Applied Microbiology, Department of Biofunctional Chemistry, Faculty of Agriculture, K

• Murakami Shuichiro

  Laboratory of Applied Microbiology, Department of Biofunctional Chemistry, Faculty of Agriculture, K

• Aoki Kenji

  Laboratory of Applied Microbiology, Department of Biofunctional Chemistry, Faculty of Agriculture, K

• Aoki K

  Department Of Applied Biological Chemistry Graduate School Of Agricultural Science Kobe University

• Takenaka Shinji

  Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University

• Murakami S

  Kikkoman Corp. Chiba Pref. Jpn

• Maeda Tetsuya

  Laboratory Of Applied Microbiology Department Of Biofunctional Chemistry Faculty Of Agriculture Kobe

• HAYASHI Teruhiko

  Laboratory of Applied Microbiology, Department of Biofunctional Chemistry, Faculty of Agriculture, K

• Murakami Shuichiro

  Dep. Of Bioscience Biotechnology And Agrochemistry Fac. Of Agriculture Meiji Univ.

• Takenaka Shinji

  Department Of Agrobioscience Graduate School Of Agricultural Science Kobe University

• Aoki K

  Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University

• Hayashi Teruhiko

  Laboratory Of Applied Microbiology Department Of Biofunctional Chemistry Faculty Of Agriculture Kobe

• Aoki Kenji

  Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University

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