Purification and Characterization of Catechol 1, 2-Dioxygenase from Aniline-assimilating Rhodococcus erythropolis AN-13
スポンサーリンク
概要
- 論文の詳細を見る
Catechol 1, 2-dioxygenase (EC 1.13.11.1) was purified to homogeneity from a cell-free extract of aniline-grown Rhodococcus erythropolis AN-13. The purified enzyme was stable between pH 7.0 and 10.5 and up to 40°C. The optimal pH was 7.5 for catechol as a substrate. The molecular weight of the enzyme was 37, 000 by gel filtration and 36, 000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This enzyme contained 3mol of sulfhydryl groups per mol of protein, but not disulfide bonds of cystine residues. The enzymatic activity was inhibited by AgNO3, HgCl2, iodoacetic acid and p-chloromercuribenzoic acid. FeSO4 and FeCl3 also inhibited the activity, although the enzyme contained 1.3g atoms of iron per mol of protein. This enzyme catalyzed the intradiol cleavage of 3- and 4-methylcatechol, but not the extradiol cleavage of 3-methylcatechol.
- 社団法人 日本農芸化学会の論文
著者
-
Shinke Ryu
Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University
-
Aoki Kenji
Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University
-
Nishira Hiroshi
Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University
-
NISHIRA Hiroshi
Department of Agricultural Chemistry faculty of Agriculture, Kobe University
-
KONOHANA Tsukasa
Graduate School of Science and Technology, Kobe University
-
SHINKE Ryu
Department of Agricultural Chemistry faculty of Agriculture, Kobe University
関連論文
- Screening and Characterization of Bacteria That Can Utilize Ammonium and Nitrate Ions Simultaneously under Controlled Cultural Conditions(ENVIRONMENTAL BIOTECHNOLOGY)
- Successful Surgical Treatment of Tricuspid Valve Endocarditis Associated with Vertebral Osteomyelitis
- Constitutive Synthesis of Enzymes Involved in 2-Aminophenol Metabolism and Inducible Synthesis of Enzymes Involved in Benzoate, p-Hydroxybenzoate, and Protocatechuate Metabolism in Pseudomonas sp. Strain AP-3
- Ammonia Assimilation in Klebsiella pneumoniae F-5-2 That Can Utilize Ammonium and Nitrate Ions Simultaneously : Purification and Characterization of Glutamate Dehydrogenase and Glutamine Synthetase
- Isolation and Culture Conditions of a Klebsiella pneumoniae Strain That Can Utilize Ammonium and Nitrate Ions Simultaneously with Controlled Iron and Molybdate Ion Concentrations(Microbiology & Fermentation Technology)
- Purification and characterization of a halotolerant intracellular protease from Bacillus subtilis strain FP-133
- The Role of SH and S-S Group in Bacillus cereus β-Amylase
- Purification, Characterization, and Gene Analysis of Catechol 2,3-Dioxygenase from the Aniline-Assimilating Bacterium Pseudomonas Species AW-2
- Induction, Purification, and Characterization of Catechol 2,3-Dioxygenase from Aniline-assimilating Pseudomonas sp. FK-8-2(Biological Chemistry)
- Microbial Metabolism of Aniline through a meta-Cleavage Pathway : Isolation of Strains and Production of Catechol 2,3-Dioxygenase(Microbiology & Fermentation Industry)