Microbial Transformation of Aniline Derivatives : Regioselective Biotransformation and Detoxification of 2-Phenylenediamine by Bacillus cereus Strain PDa-1(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
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A bacterial isolate, strain PDa-1, grew well on basal medium supplemented with 2-phenylenediamine, sucrose, and ammonium nitrate and completely transformed 2-phenylenediamine. The isolate was identified as Bacillus cereus. The product formed from 2-phenylenediamine was identified by EI-MS and NMR as 2-aminoacetanilide; whole cells converted 2-phenylenediamine to the product with a 76% molar yield. Whole cells also showed a broad substrate specificity toward 20 of 26 tested arylamines with substituent groups of various size and positions. Especially 2-aminobenzoic acid, 4-aminosalicylic acid, 5-aminosalicylic acid, and 2-aminofluorene were converted completely to the corresponding product with an aminoacetyl group. Cell extracts of strain PDa-1 had a high arylamine N-acetyltransferase activity. The partially purified enzyme converted 2-phenylenediamine to 2-aminoacetanilide. Strain PDa-1 constitutively expressed the enzyme in the absence of 2-phenylenediamine. Effects of 2-phenylenediamine and 2-aminoacetanilide on growth indicated that this enzyme probably plays a role in the detoxification of toxic arylamines in this strain.
- 社団法人日本生物工学会の論文
- 2006-07-25
著者
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Aoki K
Department Of Applied Biological Chemistry Graduate School Of Agricultural Science Kobe University
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Takenaka Shinji
Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University
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MURAKAMI Shuichiro
Department of Bioscience, Biotechnology, and Agrochemistry, Faculty of Agriculture, Meiji University
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AOKI Kenji
Department of Nutritional Management, Faculty of Nutritional Science, Sagami Women’s University
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Takahashi Yuka
Department of Hygiene and Public Health of Osaka Medical College
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Murakami S
Kikkoman Corp. Chiba Pref. Jpn
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Takahashi Yuka
Department Of Biofunctional Chemistry Faculty Of Agriculture Kobe University
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Sasano Yasuhiro
Division of Biosystem Chemistry, Graduate School of Science and Technology, Kobe University
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Sasano Yasuhiro
Department Of Biosystems Science Division Of Biosystems Chemistry Graduate School Of Science And Tec
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Murakami Shuichiro
Dep. Of Bioscience Biotechnology And Agrochemistry Fac. Of Agriculture Meiji Univ.
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Aoki Kenji
Department Of Nutritional Management Faculty Of Nutritional Science Sagami Women's University
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Takenaka Shinji
Department Of Agrobioscience Graduate School Of Agricultural Science Kobe University
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Aoki K
Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University
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Murakami Shuichiro
Department Of Bioscience Biotechnology And Agrochemistry Faculty Of Agriculture Meiji University
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Aoki Kenji
Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University
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MURAKAMI Shuichiro
Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University
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