Purification and Characterization of Two Novel Halotolerant Extracellular Proteases from Bacillus subtilis Strain FP-133
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概要
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Bacillus subtilis strain FP-133, isolated from a fermented fish paste, synthesized two novel halotolerant extracellular proteases (expro-I and expro-II), showing activity and stability at concentrations of 0–20% (w/v) NaCl. Each protease was purified to homogeneity and characterized. The purified expro-I was a non-alkaline serine protease with an optimum pH of 7.5, although most serine proteases from Bacillus strains act at the alkaline side. The molecular mass of expro-I was 29 kDa. The purified expro-II was a metalloprotease with a molecular mass of 34 kDa. It was activated by Fe2+, which has never been reported as a bacterial protease activator. At a concentration of 7.5% (w/v) NaCl, both proteases preferred animal proteins to vegetable proteins as natural substrates. In addition, under saline conditions, expro-I and II showed high catalytic activity toward gelatin and casein respectively.
- 社団法人 日本農芸化学会の論文
- 2006-02-23
著者
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Takenaka Shinji
Laboratory of Applied Microbiology, Department of Biofunctional Chemistry, Faculty of Agriculture, K
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Murakami Shuichiro
Laboratory of Applied Microbiology, Department of Biofunctional Chemistry, Faculty of Agriculture, K
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Aoki Kenji
Laboratory of Applied Microbiology, Department of Biofunctional Chemistry, Faculty of Agriculture, K
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Aoki K
Department Of Applied Biological Chemistry Graduate School Of Agricultural Science Kobe University
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Takenaka Shinji
Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University
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SETYORINI Endang
Division of Science of Biological Resources, Graduate School of Science and Technology, Kobe Univers
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Setyorini Endang
Division Of Life Science Graduate School Of Science And Technology Kobe University
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Murakami S
Kikkoman Corp. Chiba Pref. Jpn
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Murakami Shuichiro
Dep. Of Bioscience Biotechnology And Agrochemistry Fac. Of Agriculture Meiji Univ.
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Takenaka Shinji
Department Of Agrobioscience Graduate School Of Agricultural Science Kobe University
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Aoki K
Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University
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Aoki Kenji
Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University
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