Induction, Purification, and Characterization of Catechol 2,3-Dioxygenase from Aniline-assimilating Pseudomonas sp. FK-8-2(Biological Chemistry)

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概要

• 論文の詳細を見る

• Catechol 2,3-dioxygenase (EC 1.13.1.2) was induced by aniline in cells of the aniline-assimilating Pseudomonas sp. FK-8-2 isolated from soil. The addition of polypepton besides aniline to the incubation mixture promoted the induction of this enzyme. The enzyme was purified to homogeneity with the high recovery of 95% from a cell-free extract of anilin-grown Pseudomonas sp. FK-8-2. The purified enzyme was stable between pH 60. and 7.0 and up to 70℃ in the presence of 5% (v/v) acetone, which protected this enzyme from inactivation by air and other oxidizing agents. The molecular weight of the enzyme was 120,000 by gel filtration. It was dissociated into four identical subunits with the molecular weight of 33,000 on a sodium dodecyl sulfate-polyacrylamide gel. This enzyme catelyzed the extradiol cleavage of 3- and 4-chlorocatechol, 3- and 4-methylcatechol, and 3-fluorocatechol.

• 社団法人日本農芸化学会の論文
• 1991-05-23

著者

• Aoki K

  Department Of Applied Biological Chemistry Graduate School Of Agricultural Science Kobe University

• AOKI Kenji

  Department of Nutritional Management, Faculty of Nutritional Science, Sagami Women’s University

• Nakanishi Yoko

  Division Of Science Of Biological Resources Graduate School Of Science And Technology Kobe Universit

• Nakanishi Y

  Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University

• MURAKAMI Shuichiro

  Division of Science of Biological Resources, The Graduate School of Science and Technology Kobe Univ

• SHINKE Riu

  Department of Agricultural Chemistry, Faculty of Agriculture, Kobe University

• Shinke R

  Laboratory Of Bio-functional Chemistry Division Of Bioscience The Graduate School Of Science And Tec

• Shinke Ryu

  Division Of Science Of Biological Resources The Graduate School Of Science And Technology

• Shinke Riu

  Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University:(present Office)research

• Murakami Shuichiro

  Dep. Of Bioscience Biotechnology And Agrochemistry Fac. Of Agriculture Meiji Univ.

• Aoki Kenji

  Department Of Nutritional Management Faculty Of Nutritional Science Sagami Women's University

• Shinke Ryu

  Laboratory Of Bio-functional Chemistry Division Of Bioscience The Graduate School Of Science And Tec

• Aoki Kenji

  Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University

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