7.血清中の抗補体性因子について(第3回補体シンポジウム I 補体成分の基礎的研究)

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It has been recognized that the lack of knowledgeds on C'-inhibitor makes hazard in the study of tumor immunity as well as in that of transplantation immunity. This report deals with the results of the separation of anticomplementary substances (not antibody, but C'-inhibitors) in the sera from rbbits and C3H/He mice, and also their characterization. Separation of the sera. The anticomplementary activity in these aera were evidenced in two parts of fraction on gel-filtration with Sephdex G-200. The first part is approximately correspondent to the first peak of the serum protein fractionated with Sephdex G-200 and the second to the forth peak. The former is tentatively named Fr. I and the latter is Fr. II. Both fractions exhibit the anticomplementary effects mostly through the inhibition of C'3 activity, but the exact component of C'3 is still unknown. The properties of the Fr.I. The fr. I. The Fr.I is non-permeable through cellophane membrane, stable at 56℃ for 90min., precipitated at pH 5.5 with i.s., 0.001M NaC1 equivalent, and not inactivated through absorption with EA cells. The anticomplementary activity by incubation with GPC, is more strengthened at 37℃ than at 0℃ and the longer the stronger as far as time is concerned. The properties of the Fr. II. The Fr. II is permeable through cellophane membrane, stable at 56℃ for 90 min., and not inactivated through mixture with EA cells, but inactivated through lyophilization. The Fr. II immediately acts on C' while the Fr. I dose rather slowly as described above. The anticomplementary activity of Fr. II is eluted from DEAE cellulose column at pH 7.7, i.s. 0.045.
日本アレルギー学会の論文
1966-11-30

7.血清中の抗補体性因子について(第3回補体シンポジウム I 補体成分の基礎的研究)

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