6.C'3c Deficient Serum(第3回補体シンポジウム I 補体成分の基礎的研究)

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Sixty-nine cases of 2321 blood donors showed less than 20 unit of C'H50. After a period of one or two months, 22 cases of these 69 cases were reexamined and all of the 22 cases again showed complement titer lower than 20 C'H_<50>. Ten cases out of this low complement group showed extremely low hemolytic activity, less than one unit. It was found out that they were apparently in good health and not relate to age, sex or blood type. The ten sera with low hemolytic activity revealed extremely low C'IA50 activity, less than 1:9 (except one which revealed 1:30) in comparison with that of normal control which produced the same patterns at the dilution of either 1:4000 or 1:6000. Reactivity of each nine components of complement of these sera was measured, emplying functionally pure guinea pig components of complement. The results indicated that reactivity of C'3c was strikingly low as compared with the control sera. More endeavour was made to determine whether C'3c low reactivity was due to deficiency in C'3c or to presence of larger amount of C'3c inhibitor in the test serum. We recognized that there was no difference in inhibitory activity of C'3c reactivity as measured by immune adherence between 5 normal sera and 5 low hemolytic activities. The sera, one from normal and another from low hemolytic activity, were applied to DEAE cellulose column in order to measure the amount of C'3c and its inhibitor in separated form. The result indicated that remarkably low reactivity of C'3c was due to C'3c deficiency itself but not to its inhibitor. Immunoelectrophoretical analysis of these sera was carried out in collaboration with Matsuhashi and Usui. Rabbit anti human β1c antiserum was prepared after Matsuhashi's method. The precipitin line developed in all 10 cases of these C'3c deficient sera as well as in normal sera, and essentially no difference could be observed. As compared with precipitin lines developed with rabbit antiwhole human serum antiserum, these precipitin lines correspond to β1c globulin and this was confirmed by conversion pattern of β1c to β1_A globulin as observed in both C'3c deficient and normal sera. Recently Muller-Eberhard stated C'3 is synonymous with β1c gloulin, but from these observations, we could not conclude that C'3c was synonymous with β1c gloulin(or C'3 of Muller-Eberhard).
日本アレルギー学会の論文
1966-11-30

6.C'3c Deficient Serum(第3回補体シンポジウム I 補体成分の基礎的研究)

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