1.補体第1成分の電気泳動的解析(第3回補体シンポジウム I 補体成分の基礎的研究)

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Guinea pig C'1 has been separated into three activities as define hemolytic spots on the polyacrylamide gel by our immunolyso-electrophoresis tcchnique. All the three activities, i.e. the fast(F), medial(M) and slow(S) moving spots were demonstrated by double layer method of agar-DEAE dextran gel mixtures containing EAC'4 cell and pa. tially purified C'2, and EDTA-C'. But, in the system usintg EA cell, Sspct alone was observed. The electrophoretic run C'1 treated with 10^3 M to 10^<-2> M EDTA resulted in the marked activity of F spot and dsiappearance of M and spots. By Sephadex G-200 chromatography and sucrose density gradient centrifugation of purified C'1, the obtained were two C'1 activity peaks in which the heavy molecular fraction comprised mainly S and M and the light on mainly M and F in electrophoresis. After centrifugation of EDTA-treated C'1, in the presence of EDTA, C'1 activity was shown as single peak which was found in a similar position of light molecular faction of intact C'1. Recalcified C'1 after the treatment of EDTA was separated into two activty peaks, both composed of F and M, by centrifugation. But the recalcified C'1 could not form EAC'1 instead of C'1. Subcomponents of guinea pig C'1 could be obtaned by DEAE-cellulose column chromatography followed by Lepow's method. The eluate at ionic strength of 0.14M relative NaC1 (probably corresponding to Lepow's human C'1s) ran at the position of F spot in electrophoresis. When the fraction corresponding to Lepow's C'1q was added into detection system containing EA cell, weak M spot was revealed on the gel but F spot was not. From the above observations, it is suggested that there are different active states of C'1, e.g. a complete macromolecular state which might react with EAC'4 as well as EA cell, and incomplete macromolecular states which might react with EAC'4 cell, but not with EA cell.
日本アレルギー学会の論文
1966-11-30

1.補体第1成分の電気泳動的解析(第3回補体シンポジウム I 補体成分の基礎的研究)

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