5.ヒト補体第4成分の免疫電気泳動像について(第3回補体シンポジウム I 補体成分の基礎的研究)

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By the use of the immunoelectrophoresis, the situation of the isolated C'4 was determined, and the changes of C'4 resulting from various treatments were investigated. In addition to that, relation between the change of C'4 activity of the various patients' sera and that of the precipitin line of C'4 was examined. The precipitin line corresponding to C'4 by immunoelectrophoresis revealed a single line which migrates from the original point to andoal side in β1-globulin region. To examine the precipitin line of C'4 in the whole human serum. Ouchterlony double diffusion method was also used. With these methods, this line was correspond with β 1_E-globulin. As it is difficult to detect the β1_E-and β1c/1_A line in the whole human serum, an absorbed antihuman horse serum was used. This anti-human hourse serum (produced by the Research Foundation for microbial Disease, Osaka University) was absorbed with the whole human serum from which β1_E-and β1c/1_A globulin fraction were eliminated by chromatography. Using this antiserum, the β1_E-and β1c/1_A lines were easily detected. Under the hydrazine treatment of C'4, the β1_E line extends to cathodal side with the times of this process and gets across the β1c line, but it was no change on anodal side. About the effect of immune precipitate on C'4, this treatment results that the β1_E line gets across the β1c line, but the transfer of the β1_E line to cathodal or anodal side was not investigated. The length and density of C'4 line decreased with dilution of C'4 by a saline solution, but no changes of the situation and form were observed by this treatment. And the β1_E line vanished when the activity of C'4 became one-fourth of normal value. It was recognized that the alternation of the β1_E line in the various patients' sera kept pace with C'4 activity of these sera.
日本アレルギー学会の論文
1966-11-30

5.ヒト補体第4成分の免疫電気泳動像について(第3回補体シンポジウム I 補体成分の基礎的研究)

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