8.モルモット血清補体 C'3d について(第3回補体シンポジウム I 補体成分の基礎的研究)

概要

論文の詳細を見る
The kinetic analysis of-sites formation (SAC' 1, 4, 2, 3c , 3b, 3e, 3f, 3a, 3d) from a-sites (SAC' 1, 4, 2, 3c, 3b, 3e, 3f, 3a) and C'3d and their lysis show that the formation of d-sites is rather rapid even at 0℃ and the final values reacted are indepenedlnt of the formation of-sites is rather rapid even at 0℃ and the final values reached are indepenedlnt of the concentration of C'3d added at 0℃ and 30℃, At 37℃, however, the final values of d-sites formed depend on the concen tration of CC'3d. This difference seems to be due to that of decay rate of a-site at different temperature. The d-site does not decay at 30℃. The plotting of the reciprocal of initial velocity aganist that of a-cell concentration used (Lineweaver-Burk's plot) shows a straight line. Those data suggest the enzymic nature of C'3d. However, C'3d can clearly be depleted from the liquid phase by a-cells and the amount of removed C'3d is proportional to the concentration of a-cell added. C'3d preparations used in these experiments are fairly pure as judged by means of immunoelecttrophoresis combined with the spot lysis of cells in agar.
日本アレルギー学会の論文
1966-11-30