短日点灯条件下ゴールデンハムスター精巣において Smad2 と Smad3 は精母細胞の核内へ移行する

概要

論文の詳細を見る
The expressions of the Smad family, which transmit transforming growth factor-beta (TGF-β) superfamily in cytoplasm, have been shown in previous studies, but the biological functions of the Smad in spermatogenesis remain to be elucidated. In the present study we examine the testicular localization of Smad2 and Smad3 involved in the intracellular signal transduction of activin and transforming growth factor-beta (TGF-β) under the influence of long and short photoperiods in Syrian hamsters. Immunohistochemistry detected both Smads in Leydig cells and the cytoplasm of pachytene spermatocytes in the active testis exposed to a long photoperiod. In the regressed testis exposed to a short photoperiod, both Smads proteins were detected in Leydig cells and accumulated in the nucleus of pachytene spermatocytes. Northern blots revealed that Plasminogen Activator Inhibitor-1 (PAI-1) mRNA, an index of the transduction of TGF-β and Smads, was expressed at high levels in the photoperiod-induced regressed testis. Therefore, these Smads might participate in the signal transductions of TGF-β in the pachytene spermatocytes of photoperiod-induced testis.
日本繁殖生物学会の論文
2002-02-01