Development of a Diagnostic Method Applicable to Various Serotypes of Hantavirus Infection in Rodents
スポンサーリンク
概要
- 論文の詳細を見る
Antigenic diversity among different hantaviruses requires a variety of reagents for diagnosis of hantavirus infection. To develop a diagnostic method applicable to various hantavirus infections with a single set of reagents, we developed an enzyme-linked immunosorbent assay (ELISA) using recombinant nucleocapsid proteins of three hantaviruses, Amur, Hokkaido, and Sin Nombre viruses. This novel cocktail antigen-based ELISA enabled detection of antibodies against Hantaan, Seoul, Amur, Puumala, and Sin Nombre viruses in immunized laboratory animals. In wild rodent species, including Apodemus, Rattus, and Myodes, our ELISA detected antibodies against hantaviruses with high sensitivity and specificity. These data suggest that our novel diagnostic ELISA is a useful tool for screening hantavirus infections and could be effectively utilized for serological surveillance and quarantine purposes.
- 日本獣医学会の論文
著者
-
Kariwa Hiroaki
Laboratory Of Public Health Department Of Environmental Veterinary Sciences Graduate School Of Veter
-
ARIKAWA Jiro
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Hokkaido University
-
Takashima Ikuo
Laboratory Of Public Health Department Of Environmental Veterinary Sciences Graduate School Of Veter
-
Tkachenko Evgeniy
Chumakov Institute of Polyomyelitis and Viral Encephalitidis
-
Morozov Vyacheslav
Medical Company "Hepatolog" Incorporated
-
YOSHII Kentaro
Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University
-
YOSHIMATSU Kumiko
Department of Microbiology, Hokkaido University Graduate School of Medicine
-
Kariwa H
Hokkaido Univ. Sapporo Jpn
-
SANADA Takahiro
Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Kita–18 Nishi–9, Kita-ku, Sapporo, Hokkaido 060–0818, Japan
-
SAASA Ngonda
Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Kita–18 Nishi–9, Kita-ku, Sapporo, Hokkaido 060–0818, Japan
-
YOSHIKAWA Keisuke
Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Kita–18 Nishi–9, Kita-ku, Sapporo, Hokkaido 060–0818, Japan
-
SETO Takahiro
Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Kita–18 Nishi–9, Kita-ku, Sapporo, Hokkaido 060–0818, Japan
-
IVANOV Leonid
Plague Control Station of Khabarovsk, Sanitarny Lane, Khabarovsk 680031, Russia
-
IVANOV Leonid
Plague Control Station of Khabarovsk
-
MOROZOV Vyacheslav
Medical Company "Hepatolog" Incorporated
-
ARIKAWA Jiro
Department of Microbiology, Graduate School of Medicine, Hokkaido University
-
YOSHII Kentaro
Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University
-
SANADA Takahiro
Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University
-
SETO Takahiro
Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University
-
YOSHIMATSU Kumiko
Department of Microbiology, Graduate School of Medicine, Hokkaido University
-
NGONDA Saasa
Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University
-
TAKASHIMA Ikuo
Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Kita–18 Nishi–9, Kita-ku, Sapporo, Hokkaido 060–0818, Japan
関連論文
- ハンタウイルス感染症の疫学
- ユーラシア大陸北東部の野鼠類に寄生するBabesia microtiの疫学調査(公衆衛生学)
- Early Mortality Following Intracerebral Infection with Tick-Borne Encephalitis Virus Oshima Strain in a Mouse Model
- Generation of congenic mouse strains by introducing the virus-resistant genes, Mx1 and Oas1b, of feral mouse-derived inbred strain MSM/Ms into the common strain C57BL/6J
- Characterization and epitope mapping of monoclonal antibodies to the nucleocapsid protein of severe acute respiratory syndrome coronavirus
- スナネズミへの接種によるPuumalaおよびPuumala関連ウイルスの効率的な回収方法の開発(公衆衛生学)
- 50 C6/36細胞(ヒトスジシマカ由来)のJNKの活性化はアポトーシスを抑制する
- A06 ヒトスジシマカ由来の C6/36 細胞におけるウエストナイルウイルスの感染防御 : ウイルス感染における JNK シグナル伝達の役割
- In vitroとIn vivoにおけるラクトフェリンとリバビリンのハンダウイルスに対する抗ウイルス活性の特徴
- 血清除去による持続感染細胞中におけるボルナ病ウイルス転写量の増加
- Capture ELISA法による野外ラットにおけるボルナ病ウイルス抗体の検索
- コンタミネーションしたプラスミドを増幅することなくボルナ病ウイルスのRNAのみを検出できるRT-nested PCR法の開発(短報)
- コンタミネーションしたプラスミドを増幅することなく, ボルナ病ウイルスのRNAだけを検出できるRT-nested PCR法の開発
- Predicting the Antigenic Structure of the Pandemic (H1N1) 2009 Influenza Virus Hemagglutinin
- Disease constraints for utilization of the African buffalo (Syncerus caffer) on game ranches in Zambia
- Ecology and epidemiology of anthrax in cattle and humans in Zambia
- Characterization of H3N6 avian influenza virus isolated from a wild white pelican in Zambia
- ダニ媒介性脳炎 (特集 ヒトと家畜・ペット・野生動物の感染症--口蹄疫から学ぶ) -- (野生動物と動物由来感染症)
- Virological surveillance and phylogenetic analysis of the PB2 genes of influenza viruses isolated from wild water birds flying from their nesting lakes in Siberia to Hokkaido, Japan in autumn
- A vaccine prepared from a non-pathogenic H5N1 influenza virus strain from the influenza virus library conferred protective immunity to chickens against the challenge with antigenically drifted highly pathogenic avian influenza virus
- Construction of an infectious cDNA clone for Omsk hemorrhagic fever virus, and characterization of mutations in NS2A and NS5
- Genetic and antigenic analyses of a Puumala virus isolate as a potential vaccine strain
- Experimental Chlamydia psittaci Infection of Japanese Quail
- Polymerase Chain Reaction for the Detection of Chlamydia psittaci in the Feces of Budgerigars
- Characterization of the Mode of Hantaan Virus Infection in Adult Mice Using a Nested Reverse Transcriptase Polymerase Chain Reaction: Transient Virus Replication in Adult Mice
- Production of recombinant Hantavirus nucleocapsid protein expressed in silkworm larvae and its use as a diagnostic antigen in detecting antibodies in serum from infected rats
- Evidence for the existence of Puumula-related virus among Clethrionomys rufocanus in Hokkaido, Japan
- Characterization of the mode of Hantaan virus infection in adult mice using a nested reverse transcriptase polymerase chain reaction : transient virus replication in adult mice
- Comparison of nucleotide sequences of M genome segments among Seoul virus strains isolated from Eastern Asia
- Enhancement of infectivity of hantavirus in cell culture by
- ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF ANTIBODY AGAINST HEMORRHAGIC FEVER WITH RENAL SYNDROME VIRUS IN SERA FROM EXPERIMENTALLY INFECTED RATS
- LABELED AVIDIN-BIOTIN ENZYME-LINKED IMMUNOSORBENT ASSAY (LAB-ELISA) FOR DETECTION OF JAPANESE ENCEPHALITIS ANTIBODY IN SWINE SERA
- Distribution and characterization of tick-borne encephalitis viruses from Siberia and far-eastern Asia
- Single point mutation in tick-borne encephalitis virus prM protein induces a reduction of virus particle secretion
- 日本と極東ロシアのダニ媒介性脳炎ウイルスの系統解析と病原性
- The Mechanism of Actinomycin D-Mediated Increase of Borna Disease Virus(BDV) RNA in Cells Persistently Infected by BDV
- Phylogenetic and virulence analysis of tick-borne encephalitis viruses from Japan and far-eastern Russia
- Establishment of an Enzyme-Linked Immunosorbent Assay for Detection of Hantavirus Antibody of Rats Using a Recombinant of Nucleocapsid Protein Expressed in Escherichia coli
- 齧歯類とハンタウイルス感染症 (特集 家畜と野生動物における人と動物の共通感染症)
- Detection of Hantaviral Antibodies among Patients with Hepatitis of Unknown Etiology in Japan
- Evaluation of the potency, optimal antigen level and lasting immunity of inactivated avian influenza vaccine prepared from H5N1 virus
- Development of vaccine strains of H5 and H7 influenza viruses
- ハンタウイルス感染症 (特集 ウイルス性出血熱)
- Discrimination of West Nile Virus and Japanese Encephalitis Virus Strains Using RT-PCR RFLP Analysis
- 野外のコンベンショナル豚を用いた豚繁殖・呼吸障害症候群ウイルス(PRRSV)の抗体測定におけるEnzyme-Linked Immunosorbent Assay(ELISA)とImmunofluorescent Antibody(IFA)の評価(ウイルス学)
- ブニヤウイルス : ウイルスと宿主の相互関係・ハンタウイルスとげっ歯類の共進化
- アルボウイルス感染症の疫学
- ハンタウイルス (特集 小児の感染症(1)) -- (ウイルス)
- 新しいハンタウイルス感染症
- Establishment of a neutralization test involving reporter gene-expressing virus-like particles of tick-borne encephalitis virus
- Epidemiological Study of Hantavirus Infection in the Samara Region of European Russia
- Antigenic structure of the hemagglutinin of H9N2 influenza viruses.
- Mode of Infection of Hokkaido Virus (Genus Hantavirus) among Grey Red-Backed Voles, Myodes rufocanus, in Hokkaido, Japan
- Epizootiological and Epidemiological Study of Hantavirus Infection in Japan
- Comparison of virulence of various hantaviruses related to hemorrhagic fever with renal syndrome in newborn mouse model
- Epizootiological survey of hantavirus among rodent species in Ningxia Hui Autonomous Province, China
- Inactivation of SARS coronavirus by means of povidone-iodine, physical conditions, and chemical reagents
- Epizootiological Study of Tick-Borne Encephalitis Virus Infection in Japan
- Single-step reverse transcriptase-polymerase chain reaction for detection of Borna disease virus RNA in vitro and in vivo
- A single-tube RT-PCR method for the detection of Borna disease viral genomic RNA
- Viral envelope protein glycosylation is a molecular determinant of the neuroinvasiveness of the New York strain of West Nile virus
- Genetic and biological comparision of tick-borne encephalitis viruses from Hokkaido and Far-Eastern Russia
- Cleavage of the NS2-3 protein in the cells of cattle persistently infected with non-cytopathogenic bovine viral diarrhea virus
- Epidemiology and Epizootiology of Hantavirus Infection in Japan
- Molecular Epidemiological and Serological Studies of Hantavirus Infection in Northern Vietnam
- Analysis of severe acute respiratory syndrome coronavirus structural proteins in virus-like particle assembly
- An H5N1 highly pathogenic avian influenza virus that invaded Japan through waterfowl migration
- Puumala virus infection in Syrian hamsters (Mesocricetus auratus) resembling hantavirus infection in natural rodent hosts
- Application of Truncated Nucleocapsid Protein (N) for Serotyping ELISA of Murinae-Associated Hantavirus Infection in Rats
- Characterization of Monoclonal Antibodies against Hokkaido Strain Tick-Borne Encephalitis Virus
- Laoratory of Public Health (An Introduction of the four Departments composing the Graduate Scholl of Veterinary Medicine, Hokkaido University (2))
- Construction of a replicon and an infectious cDNA clone of the Sofjin strain of the Far-Eastern subtype of tick-borne encephalitis virus
- Development of a Diagnostic Method Applicable to Various Serotypes of Hantavirus Infection in Rodents
- Studies on Hantavirus Infection in Small Mammals Captured in Southern and Central Highland Area of Vietnam
- Development of an ELISA system for tick-borne encephalitis virus infection in rodents
- Needle-free jet injection of DNA and protein vaccine of the Far-Eastern subtype of tick-borne encephalitis virus induces protective immunity in mice
- Epidemiology of Hantavirus Infection in Thousand Islands Regency of Jakarta, Indonesia
- Reverse Transcription Nested Polymerase Chain Reaction for Detecting p40 RNA of Borna Disease Virus, without Risk of Plasmid Contamination.
- Distinct genetic characteristics of Sri Lankan Rattus and Bandicota (Murinae, Rodentia) inferred from mitochondrial and nuclear markers