Application of Truncated Nucleocapsid Protein (N) for Serotyping ELISA of Murinae-Associated Hantavirus Infection in Rats
スポンサーリンク
概要
- 論文の詳細を見る
Truncated recombinant nucleocapsid proteins (trNs) that lack N-terminally located cross-reactive epitopes of four Murinae rodent-associated hantaviruses, Seoul virus (SEOV), Thailand virus, Hantaan virus (HTNV) and Dobrava-Berglade virus, were produced by using a baculovirus expression system. ELISA with the trNs as antigens enabled serotyping of immune sera from rats experimentally inoculated with the corresponding hantaviruses as cut-off OD values of 60% to those to whole N of HTNV. The trN based ELISA could serotype 12 out of 13 sera obtained from wild rodent (Rattus norvegicus) naturally infected with SEOV by the 60% cut-off value. These results indicate that screening with whole N followed by serotyping with trNs as cut-off OD value of 60% to that with whole N is an useful method for serological surveillance of Murinae-associated hantavirus infection among rodents.
著者
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ARIKAWA Jiro
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Hokkaido University
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Isozumi Rie
Department Of Pediatrics Mie University School Of Medicine
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YOSHIMATSU Kumiko
Department of Microbiology, Hokkaido University Graduate School of Medicine
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ENDO Rika
Department of Microbiology, Hokkaido University Graduate School of Medicine
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SHIMIZU Kenta
Department of Microbiology, Hokkaido University Graduate School of Medicine
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YASUDA Shumpei
Department of Microbiology, Hokkaido University Graduate School of Medicine
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KOMA Takaaki
Department of Microbiology, Hokkaido University Graduate School of Medicine
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Yasuda Shumpei
Division Of Bioscience Graduate School Of Environmental Earth Science Hokkaido University
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ARIKAWA Jiro
Department of Microbiology, Graduate School of Medicine, Hokkaido University
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ARIKAWA Jiro
Department of Microbiology, Hokkaido University Graduate School of Medicine
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YOSHIMATSU Kumiko
Department of Microbiology, Graduate School of Medicine, Hokkaido University
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ISOZUMI Rie
Department of Microbiology, Hokkaido University Graduate School of Medicine
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SHIMIZU Kenta
Department of Applied Chemistry, Chuo University
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