Reverse Transcription Nested Polymerase Chain Reaction for Detecting p40 RNA of Borna Disease Virus, without Risk of Plasmid Contamination.
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概要
- 論文の詳細を見る
Several methods for the detection of Borna disease virus (BDV) RNA have been reported, one being the reverse transcription-nested polymerase chain reaction (RT-nested PCR) method. However, due to the possibility of contamination of the cloned DNA in a reaction tube, false-positive results might be obtained by RT-nested PCR. To detect only BDV RNA without anxiety of contamination, we developed an RT-nested PCR system using "mRNA selective PCR kit". Using this system, cDNA of BDV p40 in the plasmid (up to 5 × 107 molecules) was not amplified. BDV specific sequence was amplified from total RNA (more than 50 pg) of MDCK/BDV cells, which were persistently infected with BDV. These results indicate that this mRNA selective RT-nested PCR system can specifically amplify target RNA as distinguished from plasmid contaminated.
- 公益社団法人 日本獣医学会の論文
著者
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Kariwa Hiroaki
Laboratory Of Public Health Department Of Environmental Veterinary Sciences Graduate School Of Veter
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Takashima Ikuo
Laboratory Of Public Health Department Of Environmental Veterinary Sciences Graduate School Of Veter
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Nishino Yoshii
Research Institute Of Biosciences School Of Veterinary Medicine Azabu University
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Mizutani Tetsuya
Laboratories Of Public Health Hokkaido University
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NISHINO Yoshii
Research Institute of Biosciences, School of Veterinary Medicine, Azabu University, Kanagawa 229-8501, Japan
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