内リンパ水腫と補体

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Previously, we demonstrated the existence of IgG antibody in the endolymphatic fluid. In the present study, for the determination of endolymphatic complement, we established a new system using 1.5×108 cells/ml sheep erythrocytes made up to a total volume of 50μl. The value of endolymphatic CH50 determined by this system was 3.70u/ml. Endolymphatic hydrops was induced experimentally in guinea pigs using rabbit's stria vascularis as the antigen, as previously described. In the present study, the activation system of the complement involved in endolymphatic hydrops was assessed in C4 deficient guinea pigs (C4D guinea pigs). Under anesthesia with nembutal, these animals were perfused with 10% neutral formalin and immobilized.Observations were made after HE staining. In normal guinea pigs (Hartley strain) endolymphatic hydrops was induced in 40% (4/10), but in none of the 18 C4D guinea pigs. These results suggest that the main activation system for the complement involved in the development of endolymphatic hydrops is the classical pathway.
耳鼻咽喉科臨床学会の論文