マダコ肝臓からのβ-N-アセチルヘキソサミニダ-ゼの分離とその酵素的性質

元データ 日本水産學會

概要

The liver of common octopus Octopus vulgaris contained extremely high activity of β-N-acetyl-hexosaminidase which is useful for structural and functional studies of complex carbohydrates. Asimple procedure has been devised for the isolation of β-N-acetylhexosaminidase from the liver inan electrophoretically homogeneous form. The procedure involved heat treatment at 68°C for 20min, Sephadex G-200 gel filtration, and CM-Sephadex C-50 chromatography. The purified enzyme was sufficiently free from other glycosidases and proteases. The molecular weight of the enzyme was estimated to be 147, 000 by Sephadex G-200 gel filtration. The enzyme exhibited the optimum pH at pH 5.0 and was stable between pH 4 and 8. For p-nitrophenyl β-N-acetylgluco-saminide, Km was 0.63mM and Vmax was 192μmol/min•mg.

著者

榎本 則行 佐賀大学農学部
浅川 牧夫 通宝海苔(株)
浅川 牧夫 熊本大学教育学部食物学研究室
中川 浩毅 佐賀大学農学部
中川 浩毅 佐賀大学農学部生命機能科学科
浅川 牧夫 熊本大学教育学部

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