ウナギ表皮におけるB-N-アセチルヘキソサミニダ-ゼの組織化学的検出〔英文〕

元データ 日本水産學會

概要

To elucidate the origin of β-N-acetylhexosaminidase which had been found in the external mucous materials of varlous fishes, histochemical detection of β-N-acetylhexosaminidase activlty in the epidermis of eel was attempted using naphthol AS-BI N-acetyl, β-D-glucosaminide as sub-strafe and hexazonium pararosanilin as capture reagent. Besides, the structure of eel skin was observed histochemlcally by staining with periodate-Schiff reagent and toluidine blue. β-N-Acetylhexosaminidase activity was demonstrated as red-stained granules around the mature goblet cells in the upper layer of the epidermis. From the fact that the cell membrane in the epidermis was stained with periodate-Schiff reagent and toluidine blue, the membrane was revealed to con-tain carbohydrate components, presumably complex carbohydrates. From the above findings, it was assumed that β-N-acetylhexosaminidase might be involved in catabolic collapse of the super-ficial cells of the epidermis.

著者

榎本 則行 佐賀大学農学部
浅川 牧夫 通宝海苔(株)
浅川 牧夫 熊本大学教育学部食物学研究室
中川 浩毅 佐賀大学農学部
中川 浩毅 佐賀大学農学部生命機能科学科
榎本 則行 佐賀大学農学部農芸化学科
中川 浩毅 佐賀大学農学部農芸化学科
浅川 牧夫 熊本大学教育学部食物学教室
浅川 牧夫 熊本大学教育学部

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