ウナギ体表面粘質物中のsialic acid含有糖蛋白質に関する研究-4-糖鎖部分の単離および性質について〔英文〕

概要

論文の詳細を見る
The carbohydrate moiety from sialic acid-containing glycoprotein (SA-glycoprotein) in the external mucus of Japanese eel was isolated and partially characterized. SA-glycoprotein was treated with 0.3M NaBH4 in 0.1M NaOH at 37°C for 28h. More than 80% of the carbohydrate moiety was released from SA-glycoprotein. The alkaline-reduced SA-glycoprotein was subjected to gel filtration on Sephadex G-50 and G-25, followed by ion-exchange chromatography of Dowex 50-X2 (H+) to isolate the carbohydrate moiety. The purified carbohydrate moiety migrated to the anode as a single band in paper electrophoresis with 3% pyridine-acetic acid buffer, pH 4.5. The constituents of the carbohydrate moiety were analyzed by GLC and colorimetric methods. The molecular weight was determined by means of gel filtration. The results showed that the carbohydrate moiety was a dissacharide composed of N-acetylneuraminic acid (NANA) and N-acetylgalactosaminitol (GalNAc-ol). It is likely to have the sequence NANA→GalNAc-ol.
日本水産學會の論文