ウナギ体表面粘質物中のsialic acid含有糖蛋白質に関する研究-3-アルカリ・還元処理の影響について〔英文〕

概要

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To elucidate the effect of alkaline sodium borohydride on sialic acid-containing glycoprotein (SA-glycoprotein), it was treated with 0.3M NaBH4 in 0.1 N NaOH. The carbohydrate moiety in SA-glycoprotein was released from the protein moiety by a β-elimination mechanism. Especially, on treatment at 100°C, about 84% of the carbohydrate moiety was rapidly released in the first 10 min. All of the amino sugar in the carbohydrate moiety obtained from the alkaline-reduced SA-glycoprotein was converted into N-acetylgalctosaminitol. From the results obtained, it was elucidated that SA-glycoprotein contained the carbohydrate moiety which was attached to the protein moiety by alkali-labile linkage, presumably as O-glycosides of threonine and serine residues.
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