酵素センサ-による魚の筋肉及び血中のグルコ-ス計測〔英文〕

概要

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Glucose sensor was developed and applied to the quality control of fish products and the hemochemical health assessment of fish. Glucose sensor was prepared with the combination of a glucose membrane and an oxygen probe. Glucose oxidase was covalently immobilized on a membrane prepared from cellulose triacetate, 1, 8-diamino-4-aminomethyloctane and glutaral-dehyde. The optimum operating conditions of this sensor were determined as follows; pH 7.8, temperature: 30°C, flow rate of buffer solution: 0.65ml/min.One assay could be completed within 4 min. The enzyme sensor could be used for more than 100 assays without the decrease of output current.The saline solution of fish serum (3-16 times dilution) was suitable for the determination of its glucose with this sensor. Furthermore, it was demonstrated that the amount of glucose in sazdine meat (perchloric acid extract) could be rapidly measured by the glucose sensor developed in this study.These results suggest that the sensor proposed is an economical and reliable method for analysis of glucose in the fish muscle and serum.
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