コイ血漿成分量に及ぼす試料保存時間の影響〔魚類の血液化学検査法に関する基礎的研究-6-〕

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As ?? a ?? fundamental step to settle the haemochemical assessment of health or disease in fish, the variation of plasma constituent levels on the storage time or temperature was investigated in carp Cyprinus carpio, of 500-1, 000g in weight. The blood samples were taken by heart puncture using sodium heparin as anticoagulant. After 10min centrifugation at 3, 000rpm immediately after sampling, plasma samples were extracted and divided into 11 or 12 small tubes and stored at 5°C or -20°C. One sample was pool plasma from 2 or 3 fishes. 6 Constituent levels and 7 enzyme activities were measured in plasma samples stored at 5°C or -20°C for 0, 1, 3, 7, 15, and 30 days. And further, 7 enzyne activities were measured in plasma samples stored at -20°C for 3 hours immediately after sampling. Activities fo GPT, CPK and LDH decreased quickly with the lapse fo stored time. GPT activity decreased to about 90% after 24 hours at 5°C, and to about 70% after only 3 hours at -20°C. CPK activity decreased to about 85% after 24 hours at both 5°C and -20°C. Therefore, it is desirable that these levels are mesured immediately after blood sampling. GOT and Al-P activities were stable for 7 days at 5°C, and for 30 days to -20°C. Acid P activity and levels of glucose and uric acid were stable for 15 days at 5°C. LAP activity and levels fo urea-N, total protein, ablumin and inorganic P were stable for 30 days at both 5°C and -20°C.
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