マゴイの血清成分量からみた尾柄部切断採血法の検討〔魚類の健康評価のための血液化学検査法に関する研究-1-〕

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As a fundamental step to settle the hemochemical assessment of health or disease in fish, the comparison of various constituent levels of sera sampled by heart puncture and by severing tail peduncle was investigated in carp Cyprinus carpio. Activities or levels of GOT, LDH, creatine and inorganic phosphorus were consistently higher in sera sampled by severing tail peduncle than in those by heart puncture. These differences were ascertained to be due to contamination from the muscle at cut end. Though modified tail peduncle severing method was attempted to obtain more reliable values, consistent increases in the levels were not observed compared with those by heart puncture. The tail peduncle severing method is very easy and handy, but not suitable for measurement of these constituents. No differences in the levels or activities of total protein, albumin, glucose, urea-N, total cholesterol, magnesium, creatinine, GPT, AI-P, LAP, amylase and ChE were recognized in sera sampled by both methods. Acid P activity in serum sampled by heart puncture was higher than that by tail peduncle severing. From the above results, the most suitable blood sampling method should be chosen for the constituent under consideration.
日本水産學會の論文