血清分離までの血液放置時間によるコイ血清成分量の変動〔魚類の血液化学検査法に関する基礎的研究-3-〕

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As a fundamental step to settle the haemochemical assessment of health or disease in fish, the variation of serum constituent levels on the time placed to clot was investigated in carp Cyprinus carpio. The blood collected by heart puncture was placed in three tubes, and centrifuged after keeping for 1, 3 and 6 hours at 30°C. Serum glucose level decreased with the lapse of time. The range of 6 hour-values was from 25 to 91% of 1 hour-value. In contrast, serum levels of both magnesium and inorganic phosphorus increased. The range of the former was from 109 to 273%, and that of the latter was from 114 to 489% except in one sample. These rates varied with season. It was guessed that the occasional variations of serum activities or levels of GOT, LDH, Al-P, calcium and total protein may be the result of the collapse of thrombocyte, Serum GPT activity and chloride level did not change. From the above results, it is desirable to obtain the serum as soon as possible after blood sampling to get a trustworthy value.
日本水産學會の論文