コイの血液凝固に及ぼす取り扱いの影響

概要

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In order to investigate the effect of handling stressor on blood coagulation, hematological and hemochemical parameters and plasma clotting time were studied in carp Cyprinus carpio (body weight: about 350g). Experimental fish were acclimated to laboratory condition at water temperature ranging from 18°C to 20°C for 50 days before the experimentation. Immediately after the transfer to small tank, fish were stressed by chasing about with the net for two min and recovered for four min. Before, directly after and until 48h following handling, blood samples were collected from the caudal vessels without anesthetization using syringe tipped with 20-gauge needle.The levels of hematocrit, red blood cell, hemoglobin, serum glucose and serum calcium were increased by handling, but the serum levels of total protein, magnesium, inorganic P and chloride were not affected. Though 3 plasma clotting times: plasma recalcification time, prothrombin time and activated partial thromboplastin time, were reduced rapidly, especially plasma recalcification time was affected most markedly and did not recover within 48h following handling. The decreased plasma clotting times may be due to the response of thrombocytes to increased blood catecholamines by stress.These results suggest that plasma clotting time, particularly plasma recalcification time. is useful as an indicator of stress.
社団法人日本水産学会の論文