魚類血液へのたん白計適用の検討〔魚類の血液化学検査法に関する基礎的研究-5-〕

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The hand protein refractometer which was designed for clinical examination of human blood, was tested on four species of well-fish in order to see whether or not the instrument could be applied to fish blood. The experiments were carried out on 265 sardine Sardinops melanosticta of 11-14cm in length and 11-25g in werght in Octber, on 18 red sea bream Chrysophrys major of 12cm in mean legth and 65g in weight in October and 19 fishes of 20cm in mean length and 300g in mean weight in January, on 203 yellowtail Seriola quingueradiata of 11-41cm in length and 17-1, 400g in weight in various seasons, and on 484 carp Cyprinus carpio of 20-35cm in length and 200-3, 000g in weight in various seasons. The 54 samples on only sardine were pool serum from 5 fishes. The blood was collected 24 to 30 hours after feeding from red sea bream, yellowtail and carp, and 5 days after feeding from sardine, with syringe or by senering tail peduncle. The mean value and the range of mean ±2SD of the ratio (B/A) between serum or plasma protein levels by biurea reaction (A) and hand protein regractometer (B) were 1.08 (0.397-1.20) on sardine, 1.45 (1.28-1.62) on red sea bream, 1.37 (1.14-1.60) on yellowtail and 1.36 (1.13-1.59) on carp. This ratio was not affected by serum glucose or chloride levels in physiological range, but significantly by serum lipid level. It was revealed that the readings by the hand protein refractometer require an adjuatment by species, in order to arrive at a close appeoximation to the true protein level.
日本水産學會の論文

魚類血液へのたん白計適用の検討〔魚類の血液化学検査法に関する基礎的研究-5-〕

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