酵素センサによる海藻中の修酸の定量〔英文〕

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Oxalic acid sensor was developed and applied to the determination of oxalic acid in some algae. The sensor was prepared with the combination of an oxalate oxidase membrane and an oxygen electrode. Oxalate oxidase was covalently immobilized on a membrane prepared from cellulose triacetate, 1, 8-diamino-4-aminomethyloctane and glutaraldehyde. The optimum operating condition of this sensor was determined as follows; pH: 6.0, temperature: 20°C, flow rate of buffer solution: 0.6ml/min.One assay could be completed within 3 min. The enzyme sensor could be used for more than 70 assays without the decrease of output current (standard deviation: 24μM).Algae were ground with mortar and treated with 85°C-hot water. After the water extracts were mixed with an equal amount of chroloform-methanol (1:1), the water layers were used for the de-termination of oxalic acid. The results obtained by the sensor system were fairly agreeable with those of the conventional methods.
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