Alkaline Phosphatase (AlP)活性連続計測用酵素センサの試作

概要

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Two different types of AIP sensors were prepared with immobilized enzymes and an oxygen electrode. Good relationships were obtained between the output of the sensors and AIP activities. The optimum conditions for sensor A using nucleoside phosphorylase and xanthine oxidase as a detector were as follows: pH, 8.0; temperature, 30°C; flow rate of buffer, 0.3ml/min; flow rate of substrate, 0.1ml/min; and sample volume, 10μl. Those for sensor B using glucose oxidase were pH, 7.8; temperature, 35°C; flow rate of buffer, 0.36ml/min; flow rate of substrate, 0.16ml/min; and sample volume 10μl. Under these conditions AIP activities in fish serum were determined by both sensors. Good relationships were obtained between the values determined by the sensors and the conventional methods.
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