水産物に添加された酸化防止剤の定量に関する研究-II : BHA, BHTの定量に対する水蒸気蒸留法の適用について

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Seeking a method of unequivocally determining the BHA and BHT added to the marine products, the steam-distillation method (presented by ANGLIN et al.4)) was tentatively applied to the estimation of antioxidants in the extracts obtained by Soxhlet extraction from salted-and-dried saury, the following results being obtained. By the steam-distillation method, the separation of the antioxidants can be completed far more rapidly than by the solvent-extraction method1), and estimation of BHA is carried out without any trouble, owing to the specific reactivity of 2, 6-dichloroquinonechloroimide (2, 6-D) reagent upon BHA. This method, however, is not suitable for thereby estimating BHT, since the 2, 2-dipyridyl (2, 2-DP) reagent reacts on some contaminations coming in the distillates, leading often to ambiguous results just like the case of solvent extracts descrived in the preceeding paper1).
公益社団法人日本水産学会の論文