Effects of adenine arabinoside on duck hepatitis B virus infection. Analysis of intrahepatic replicative DNA.:Analysis of Intrahepatic Replicative DNA

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We analyzed the effects of adenine arabinoside (Ara-A) on duck hepatitis B virus (DHBV) in the serum and livers of chronically infected DHBV carrier ducks. We initially studied 9 ducks, aged 5 weeks, divided into four groups. Ducks were treated for 14 days with either Ara-A 5mg/kg (2 ducks), 10mg/kg (2 ducks) or 20mg/kg (3 ducks). Two ducks were used as a control group. DNA polymerase (DNA-P) reaction products were measured in serum and showed a decrease of approximately 50% from pretreatment values in each of the treated groups, during the course of treatment.The next phase of the experiment involved 9 5-month-old ducks divided into four groups. Ducks were treated with Ara-A 20mg/kg (2 ducks), 40mg/kg (2 ducks) or 80mg/kg (3 ducks). There were two control ducks. Ara-A was given intravenously daily for 2 weeks. Serial serum samples showed a doserelated decrease of DHBV DNA during the treatment period. Three serial wedge liver biopsies were performed (pretreatment, immediately post-treatment and four weeks post-treatment). Intrahepatic DNA was extracted and analysed by the Southern blot hybridization technique. In all treated ducks there was a decrease in intrahepatic DHBV DNA.Further analysis using the hot phenol extraction technique and densitometry showed that the decrease in DHBV DNA was predominantly due to a decrease of mature replicative forms.These results suggest that Ara-A exerts its effect in DHBV infection through an inhibition of DNA polymerase activity and resultant decrease in the formation of double stranded DNA that uses the minus strand DNA template according to the DHBV replicative pathway proposed by Summers et al.
財団法人日本消化器病学会の論文

Effects of adenine arabinoside on duck hepatitis B virus infection. Analysis of intrahepatic replicative DNA.:Analysis of Intrahepatic Replicative DNA

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