Changes of HBV DNA in liver and serum by recombinant interferon treatment.

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Twenty patients with HBeAg positive chronic liver disease were given large doses of recombinant leucocyte interferon for 4 weeks. Changes of HBV DNA in livers and sera were analyzed by Southern blot-hybridization method in paired biopsies obtained before and two weeks after treatment.<BR>Analysis of hepatic HBV DNA revealed 4 bands at 4.0, 3.2, 1.95 and 1.35 kb which appeared to represent various forms of replicative HBV DNA, i.e. relaxed circular, linear, supercoiled and single stranded HBV DNA, respectively. Out of 15 cases who were positive for hepatic HBV DNA before the treatment and in whom paired biopsies were obtained, hepatic HBV DNA became negative in 4 cases after interferon treatment and in these cases serum HBV DNA turned negative and remained negative during the observation period. Among several forms of replicative viral DNA in liver tissue, supercoiled HBV DNA tended to remain even after other forms had become undetectable. There was a close correlation between hepatic and serum HBVDNA(p<0.001).<BR>These results indicate the usefulness of Southem blot-hybridization in studying the changes of intrahepatic replicative forms of HBV DNA induced by interferon. Supercoiled HBV DNA seems to play an important role in persistence of HBV infection.
社団法人日本肝臓学会の論文