Improvement of the Plaque Technique for Human Rotaviruses: Effect of Fetal Bovine Serum, Acetyltrypsin and Agar.:Effect of Fetal Bovine Serum, Acetyltrypsin and Agar
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概要
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Dilution of viruses, which were previously treated with 10μg acetyltrypsin/ml, in MEM supplemented with 2.5% fetal bovine serum caused little damage of cell monolayers resulting in the formation of discrete plaques. When agar and acetyltrypsin were added to the overlay medium at the concentrations of 0.8% and 5μg/ml, respectively, clear plaques were induced. Linear relationships were obtained between virus concentration and the number of plaques, thus the precedure described in this report might be used for reliable plaque assay of rotaviruses. Moreover, these conditions for plaque formation were successful for estimating isolated viruses, as well as standard strains.
- 久留米大学医学部 The Kurume Medical Journal 編集部の論文
著者
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Imamura Yoshihiro
Department Of Cardiology Iizuka Hospital
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Yamamoto Shigeru
Department Of Biology Faculty Of Science Kyushu University
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IMAMURA YOSHIHIRO
Department of Virology, Kuyume University School of Medicine
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SHINGU MASAHISA
Department o f Microbiology, Kurume University School of Medicine
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