Isolation and antigenic characterization of dense particles of swine enteroviruses.
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概要
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Dense particles (buoyant density in CsCl: 1.45-1.47 g/cm<SUP>3</SUP>) were isolated from HeLa cells infected with swine enteroviruses and coxsackievirus type B5. These dense particles had important properties which were different from those of poliovirus and other picornaviruses described previously; for instance, they could be isolated under various conditions of virus growth. The smallest capsid polypeptide, VP4, was not detected by SDS-PAGE analysis. The preparations of dense particles were non-infectious and showed H antigenicity by a modified enzyme immunoassay. Judging from these properties, dense particles might be equivalent to "A particles". These findings suggest that the VP4 polypeptide is responsible for the conformational stabilization of the intact virion.
- 久留米大学医学部 The Kurume Medical Journal 編集部の論文
著者
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Hamada Nobuyuki
Department Of Quantum Biology Division Of Bioregulatory Medicine And The 21st Century Center Of Exce
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SHINGU MASAHISA
Department of Microbiology, Kurume University School of Medicin
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URAKAWA TOYOHIKO
Department of Virology, Kurume University School of Medicine
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SHINGU MASAHISA
Department o f Microbiology, Kurume University School of Medicine
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