Construction of an Expression Vector for Human Papillomavirus Type 16 E7-Glutathion S Transferase Fusion Protein.
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概要
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An expression vector for human papillomavirus type 16 (HPV 16) E7-glutathion S transf erase fusion protein was constructed. The E7 gene was confirmed by southern blot analysis with a digoxygenin 11-dUTP labeled and simultaneously amplified E7 DNA probe by polymerase chain reaction (PCR). The fusion protein was expressed in E. coli, recoverd as inclusion bodies, and was confirmed by western blot analysis using a monoclonal an-tibody against HPV 16-E7 protein. A polyclonal antibody against the HPV 16-E7 protein was raised in the serum of mice hyper-immuned with the fusion protein. This antibody will be available for screening of patients with cervical cancer.
- 久留米大学医学部 The Kurume Medical Journal 編集部の論文
著者
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SHINGU MASAHISA
Department of Microbiology, Kurume University School of Medicin
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CHINAMI MASANOBU
Department of Gynecology and Obstetrics, Kurume University, School of Nedicine
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SHINGU MASAHISA
Department o f Microbiology, Kurume University School of Medicine
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