Attempts to label bovine enterovirus with 32P.
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概要
- 論文の詳細を見る
RNase Tl oligonucleotide mapping analysis using <SUP>32</SUP>P contributes to the identification and classification of various kinds of viruses. The MI-320 strain of bovine enterovirus was labelled with H<SUB>3</SUB>PO<SUB>4</SUB> in vivo for the mapping analysis. One cycle of CsCl centrifugation of the crude virus derived from 4 Roux cultures yielded a significant amout of purified virion from which the virion RNA was isolated. Agarose-acrylamide gel electrophoresis of this RNA gave a single sharp peak at a position slightly ahead of the 28 S mouse ribosomal RNA. The specific radioactivity of this virion RNA was not sufficient for the RNase Tl oligonucleotide mapping analysis.
- 久留米大学医学部 The Kurume Medical Journal 編集部の論文
著者
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Hamada Nobuyuki
Department Of Quantum Biology Division Of Bioregulatory Medicine And The 21st Century Center Of Exce
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Imamura Yoshihiro
Department Of Cardiology Iizuka Hospital
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SHINGU MASAHISA
Department of Microbiology, Kurume University School of Medicin
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HAMADA NOBUYUKI
Department of Virology, Kurume University School of Medicine
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URAKAWA TOYOHIKO
Department of Virology, Kurume University School of Medicine
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IMAMURA YOSHIHIRO
Department of Virology, Kurume University School of Medicine
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SHINGU MASAHISA
Department o f Microbiology, Kurume University School of Medicine
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