Subcloning and trial of expression of the protein kinase catalytic domain of RSV-src gene.
スポンサーリンク
概要
- 論文の詳細を見る
The gene of catalytic domain of the protein kinase of RSV-src was cloned into the BamHI cloning site of a translation vector pET-8c which containing T7 RNA polymerase promotor, and transformed BL21 (DE3) pLys S (Studier and Moffatt, 1986). The putative molecular weight of the protein was about 33 kd as evaluated on the basis of its nucleotide size showed the identical mobility in SDS-polyacrylamide gel electrophoresis. However, yield of protein production was not high, probably, because of its instability in Escherichia coli.
- 久留米大学医学部 The Kurume Medical Journal 編集部の論文
著者
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Yuge Kentaro
Department of Cardiology Regeneration&Advanced medical Science, Graduate School of Medicine, Gifu Un
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OHTSU Yasushi
Department of Virology, Kurume University School of Medicine
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Ohtsu Yasushi
Department Of Virology Kurume University School Of Medicine
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Goto Masashi
Department Of Comprehensive Care And Education Tenri Hospital
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Chinami Masanobu
Department Of Nutritional Science Kyushu Women's University
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SHINGU MASAHISA
Department of Microbiology, Kurume University School of Medicin
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YUGE KENTARO
Department of Virology, Kurume University School of Medicine
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KUMASHIRO HIROMICHI
Department of Virology, Kurume University School of Medicine
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CHINAMI MASANOBU
Department of Virology, Kurume University School of Medicine
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CHINAMI MASANOBU
Department of Gynecology and Obstetrics, Kurume University, School of Nedicine
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SHINGU MASAHISA
Department o f Microbiology, Kurume University School of Medicine
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Goto Masashi
Department of Applied Chemistry, Faculty of Engineering, Nagoya University
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