Identification of a Novel Planarian G-Protein-Coupled Receptor That Responds to Serotonin in Xenopus laevis Oocytes
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概要
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Planarians are useful animals for regenerative and neuroscience research at the molecular level. Previously, we have reported the distribution and function of neurotransmitter-synthesizing neurons in the planarian central nervous system. In order to understand the neural projections and connections, it is important to understand the distribution of neurotransmitter receptors. In this study, we isolated a serotonin receptor gene and named it DjSER-7 (Dugesia japonica serotonin receptor type 7). DjSER-7-expressing cells were distributed in the planarian brain. According to electrophysiological analysis using Xenopus oocytes, current response was detected upon exposure to serotonin, but not other neurotransmitters in oocytes that were co-injected with mRNAs of both DjSER-7 and Gα chimera B-2, which can interact with either Gq-, Gs- or Gi-coupled receptor. In contrast, current response was not detected after exposure to neurotransmitters in oocytes injected with only DjSER-7 mRNA. Our results indicated that DjSER-7 responds to serotonin, as indicated by electrophysiological analysis using Xenopus oocytes.
著者
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Otomo Jun
Central Research Laboratory, Hitachi, Ltd.
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NISHIMURA Kaneyasu
Department of Neurobiology, Kyoto Pharmaceutical University
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Kitamura Yoshihisa
Department Of Neurobiology Kyoto Pharmaceutical University
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Agata Kiyokazu
Department Of Biology Faculty Of Science Okayama University
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Otomo Jun
Central Research Laboratory Hitachi Ltd.:(present Address)life Science Group Hitachi Ltd.
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Kaneko Shuji
Department Of Applied Biological Chemistry Graduate School Of Agricultural Science Kobe University
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Taniguchi Takashi
Department Of Geophysics Faculty Of Science Hokkaido University
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Unemura Kazuhiro
Department of Neuropharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University
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Tsushima Jun
Department of Neurobiology, Kyoto Pharmaceutical University
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Yamauchi Yosuke
Department of Neuropharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University
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Yamauchi Yosuke
Department Of Mechanical Engineering Tokyo Metropolitan University
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Nakamura K
Department Of Neurobiology Kyoto Pharmaceutical University
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Taniguchi Takashi
Department Of Dermatology Faculty Of Medicine University Of Tokyo
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TANIGUCHI Takashi
Department of Computational Science and Engineering, Nagoya University
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Kitamura Yoshihisa
Department of Clinical Pharmacy, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University, Japan
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Nishimura Kaneyasu
Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University
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Taniguchi Takashi
Department of Biology, Kyoto Pharmaceutical University
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