Detoxification of 6-Hydroxydopamine-Induced Dopaminergic Neurodegeneration by 5,5-Dimethyl-1-pyrroline N-Oxide, a Radical Trapper, in Hemiparkinsonian Rats
スポンサーリンク
概要
- 論文の詳細を見る
Several useful spin trap agents have been identified for the identification and quantification of biological oxygen radicals. Among them, 5,5-dimethyl-1-pyrroline N-oxide (DMPO) has been used most frequently as a spin trap agent. The function of DMPO in vivo, however, is still unclear. Thus, the purpose of this study was to evaluate the effect of DMPO in an in vivo model of Parkinson’s disease (PD). Rats were microinjected with 6-hydroxydopamine (6-OHDA, 32 nmol) in the presence or absence of DMPO (0.4, 4 nmol). We investigated behavioral and histochemical parameters in this rat model of PD. In addition, to examine the effect of DMPO against oxidative stress, we performed an electron spin resonance (ESR) analysis. Intranigral injection of 6-OHDA alone caused behavioral dysfunction and a massive loss of tyrosine hydroxylase (TH)-positive neurons in the substantia nigra pars compacta (SNpc). Co-microinjection of 4 nmol DMPO, but not 0.4 nmol, significantly prevented 6-OHDA-induced behavioral impairments and dopaminergic neurodegeneration. In ESR analysis, DMPO directly trapped hydroxyl radical (·OH) generated from 6-OHDA and Fe2+ in a concentration-dependent manner. These results suggest that DMPO attenuates 6-OHDA-induced dopaminergic neurodegeneration in a rat model of PD via scavenging ·OH, and is a useful tool for biological research of oxidative stresses.
- 公益社団法人 日本薬学会の論文
著者
-
Ashihara Eishi
Department Of Cardiovascular Medicine Graduate School Of Medicine Kyoto Prefectural University Of Me
-
Yasui Hiroyuki
Department Of Analytical And Bioinorganic Chemistry Kyoto Pharmaceutical University
-
Takata Kazuyuki
Department Of Mechanical Engineering Aichi Institute Of Technology
-
Inden Masatoshi
Department Of Neurobiology And 21st Century Coe Program Kyoto Pharmaceutical University
-
Takeuchi Kosei
Kan Research Institute Inc. Kobe Mi R&d Center
-
Kitamura Yoshihisa
Department Of Neurobiology Kyoto Pharmaceutical University
-
Yoshimoto Kanji
Department Of Forensic Medicine Kyoto Prefectural University Of Medicine
-
Inden Masatoshi
Department of Clinical and Translational Physiology, Kyoto Pharmaceutical University
-
Takata Kazuyuki
Department of Clinical and Translational Physiology, Kyoto Pharmaceutical University
-
Yoshimoto Kanji
Department of Legal Medicine, Kyoto Prefectural Univirsity
-
Ashihara Eishi
Department of Clinical and Translational Physiology, Kyoto Pharmaceutical University
-
Kitamura Yoshihisa
Department of Clinical Pharmacy, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University, Japan
関連論文
- 赤痢アメーバ原虫に対するトリフルオロメチオニン誘導体の有効性(研究論文紹介)
- Identification of a Novel Planarian G-Protein-Coupled Receptor That Responds to Serotonin in Xenopus laevis Oocytes
- Oxidative Neurodegeneration Is Prevented by UCP0045037, an Allosteric Modulator for the Reduced Form of DJ-1, a Wild-Type of Familial Parkinson's Disease-Linked PARK7
- DJ-1 binds to mitochondrial complex I and maintains its activity
- OJ-058 Bone Marrow AT1 Receptor Augments Neointima Formation by Promoting Mobilization of Smooth Muscle Progenitor Cells in a Platelet-Derived SDF-1α-Dependent Manner(OJ10,Atherosclerosis, Basic (IHD),Oral Presentation (Japanese),The 73rd Annual Scientifi
- OE-232 Bone Marrow Angiotensin AT1 Receptor Regulates Differentiation of Monocyte/Macrophage-Lineage Progenitors from Hematopoietic Stem Cells(OE40,Atherosclerosis, Basic 2 (IHD),Oral Presentation (English),The 73rd Annual Scientific Meeting of The Japane
- PJ-376 Bone Marrow AT1 and AT2 Differentially Modulate Vascular Repair by Regulating Mobilization of Bone Marrow-derived c-Kit^-Sca-1^+Lin^- Progenitors via SDF-1 α/CXCR4 Axis(Restenosis basic/clinical(04)(IHD),Poster Session(Japanese),The 72nd Annual Sci
- OE-416 Novel Action of Angiotensin AT1 Receptor on Bone Marrow Stem Cells, Promotion of Monocyte/Macrophage Lineage Differentiation from Hematopoietic Stem Cells.(Atherosclerosis, clinical(02)(IHD),Oral Presentation(English),The 72nd Annual Scientific Mee
- Intracoronary Transplantation of Non-Expanded Peripheral Blood-Derived Mononuclear Cells Promotes Improvement of Cardiac Function in Patients With Acute Myocardial Infarction
- 5 Intracoronary Transplantation of Non-Expanded Peripheral Blood Mononuclear Cells Promotes Improvement of Cardiac Function in Patients with Acute Myocardial Infarction(Cell-Based Cardiac Repair for Heart Failure,Meet the Expert 6 (ME6) (M),The 70th Anniv
- Neuroprotective Effects of Quercetin and Rutin on Spatial Memory Impairment in an 8-Arm Radial Maze Task and Neuronal Death Induced by Repeated Cerebral Ischemia in Rats
- PJ-131 Bone Marrow AT1 and AT2 Modulate Vascular Repair by Regulating Mobilization and Differentiation of Vascular Progenitor Cells and Macrophage Activation(Restenosis basic/clinical-3, The 71st Annual Scientific Meeting of the Japanese Circulation Socie
- PJ-072 Angiotensin II Receptor Blocker Stabilizes Vulnerable Plaque and Reduces Susceptibility of Plaque Rupture in Apolipoprotein E Deficient Mice(Atherosclerosis, basic-8, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-068 A Wire-Induced Arterial Injury Accelerated the Destabilization of Remote Established Atherosclerotic Plaques in ApoE-deficient Mice(Atherosclerosis, basic-8, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PE-034 Bone Marrow AT1 Accelerated the Development of Atherosclerosis by Regulating Differentiation of Macrophage Progenitors and Macrophage Functions(Atherosclerosis, basic-4, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PE-197 Msx1 Induces Myogenic Transformation of Fibroblasts in Myocardial Infarction in Mice(Chronic coronary heart disease/Remodeling-3 (IHD) PE33,Poster Session (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- OJ-026 Angiotensin II Type2 Receptor in Bone Marrow-Derived Cells Inhibits Neointimal Hyperplasia by Counteracting Type1 Receptor Function in Mouse Wire-Injured Artery(Restenosis, basic/clinical-1 (IHD) OJ5,Oral Presentation (Japanese),The 70th Anniversar
- OE-201 Erythropoietin-Mediated Mobilization of Endothelial Progenitors Enhances Reendothelialization via Akt-eNOS activation and Prevents Neointimal Hyperplasia of Injured Arteries(Endothelium-1 (H) OE34,Oral Presentation (English),The 70th Anniversary An
- FRS-002 Angiotensin II Type 1 Receptor in Bone Marrow-Derived Stem Cells Plays a Crucial Role in Atherosclerosis Development(Frontier of Atherosclerosis Research (H) FRS1,Featured Research Session,The 70th Anniversary Annual Scientific Meeting of the Japa
- Erythropoietin Accelerates Regeneration of Endothelium and Prevents Neointimal Hyperplasia after Vascular Injury by Mobilizing Endothelial Progenitor Cells(Endothelium/NO 4 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Nicotinic Acetylcholine α4β2 Receptor Regulates the Motivational Effect of Intracranial Self Stimulation Behavior in the Runway Method
- Parkinsonian Rotenone Mouse Model: Reevaluation of Long-Term Administration of Rotenone in C57BL/6 Mice
- Chronic Treatment with Imipramine and Lithium Increases Cell Proliferation in the Hippocampus in Adrenocorticotropic Hormone-Treated Rats
- Anti-myeloma effect of homoharringtonine with concomitant targeting of the myeloma-promoting molecules, Mcl-1, XIAP, and β-catenin
- The Bcr-Abl tyrosine kinase inhibitor imatinib and promising new agents against Philadelphia chromosome-positive leukemias
- Protection Against Oxidative Stress-Induced Neurodegeneration by a Modulator for DJ-1, the Wild-Type of Familial Parkinson's Disease-Linked PARK7
- Protection Against Oxidative Stress-Induced Neurodegeneration by a Modulator for DJ-1, the Wild-Type of Familial Parkinson’s Disease-Linked PARK7
- Massive Epistaxis From a Thrombosed Intracavernous Internal Carotid Artery Aneurysm 2 Years After the Initial Diagnosis : Case Report
- Proteasome Inhibitors Protect Against Degeneration of Nigral Dopaminergic Neurons in Hemiparkinsonian Rats
- Pharmacological Characteristics of Rotational Behavior in Hemiparkinsonian Rats Transplanted With Mouse Embryonic Stem Cell-Derived Neurons
- Possible Involvement of Small Oligomers of Amyloid-β Peptides in 15-Deoxy-△^ Prostaglandin J_2-Sensitive Microglial Activation
- Studies on Somnolence in the Daytime Caused by Drugs Used for Neuropathic Pain
- The Parkinsonian Models : Invertebrates to Mammals
- Inhibitory Effects of Antiparkinsonian Drugs and Caspase Inhibitors in a Parkinsonian Flatworm Model
- バナジルーハロゲン置換ピコリン酸錯体のインスリン様作用
- Neurotrophic Effect of Hepatic Growth Factor (HGF) on Reinnervation of Perivascular Calcitonin Gene-Related Peptide (CGRP)-Containing Nerves Following Phenol-Induced Nerve Injury in the Rat Mesenteric Artery
- Effect of Diazepam on the Runway Method Using Priming Stimulation of Intracranial Self Stimulation Behavior
- The 5-HT_ Receptor Full Agonist, 8-OH-DPAT Inhibits ACTH-Induced 5-HT_ Receptor Hyperfunction in Rats : Involvement of 5-HT_ Receptors in the DOI-Induced Wet-Dog Shakes in ACTH-Treated Rats(Pharmacology)
- Increased DOI-Induced Wet-Dog Shakes in Adrenocorticotropic Hormone-Treated Rats Are Not Affected by Chronic Imipramine Treatment : Possible Involvement of Enhanced 5-HT_-Receptor Expression in the Frontal Cortex
- Developmental expression of neural Wiskott-Aldrich syndrome protein (N-WASP) and WASP family verprolin-homologous protein (WAVE)-related proteins in postnatal rat cerebral cortex and hippocampus
- Morphological Change by Overexpression of D385A Dominant Negative Presenilin 1 in Human Neuroblastoma SH-SY5Y Cells
- Effect of HSP47 on Prolyl 4-Hydroxylation of Collagen Model Peptides
- Possible Involvement of P2X_7 Receptor Activation in Microglial Neuroprotection against Focal Cerebral Ischemia in Rats(Pharmacology)
- Identification and distribution of tryptophan hydroxylase (TPH)-positive neurons in the planarian Dugesia japonica
- Distribution of DJ-1, Parkinson's Disease-Related Protein PARK7, and Its Alteration in 6-Hydroxydopamine-Treated Hemiparkinsonian Rat Brain
- Recovery of Focal Brain Ischemia-Induced Behavioral Dysfunction by Intracerebroventricular Injection of Microglia
- FUNCTIONAL ASSAY OF DOPAMINERGIC AND SEROTONINERGIC NEURONS IN PLANARIAN(Physiology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Intracerebroventricular Injection of Microglia Protects Against Focal Brain Ischemia
- Possible Involvement of Both Endoplasmic Reticulum -and Mitochondria-Dependent Pathways in Thapsigargin-Induced Apoptosis in Human Neuroblastoma SH-SY5Y Cells
- Involvement of Wiskott-Albrich Syndrome Protein Family Verprolin- Homologous Protein (WAVEAa9 and Rac1 in the Phagocytosis of Amyloid-β(1-42) in Rat Microglia
- Apoptotic Cell Death in Neurons and Glial Cells : Implications for Alzheimer's Disease
- Possible Involvement of Rapamycin-Sensitive Pathway in Bcl-2 Expression in Human Neuroblastoma SH-SY5Y Cells
- Motivational Effect of Nomifensine in the Intracranial Self-Stimulation Behavior Using a Runway Method(Pharmacology)
- Selegilin Exerts Antidepressant-Like Effects During the Forced Swim Test in Adrenocorticotropic Hormone-Treated Rats
- Motivational Effects of Methamphetamine as Measured by the Runway Method Using Priming Stimulation of Intracranial Self-stimulation Behavior(Analytical Biochemistry)
- Effects of Imipramine and Bupropion on the Duration of Immobility of ACTH-Treated Rats in the Forced Swim Test : Involvement of the Expression of 5-HT_ Receptor mRNA(Pharmacology)
- Nuclear reorganization during dedifferentiation of differentiated cells(Joint Symposium of the Society of Chromosome Research and the Chromosome Colloquium : "Dynamics of genome and chromosomes-aspects from gene expression to evolution-",Abstracts of the
- Structural Analysis of Nuclear Reprogramming during Newt Dedifferentiation Process(From Model Laboratory Species to Model Wild Species; the Next Generation of Model Animals Derived from Japan, but Applicable Worldwide: Japanese Wood Mouse, Japanese Medaka
- Hypnotic and Sleep Quality-Enhancing Properties of Kavain in Sleep-Disturbed Rats
- A New Physiologically Based Pharmacokinetic Analysis for In Vivo Organ Disposition of Nitroxyl Spin Probes in Mice as Measured by Multisite Detection L-band ESR (MSD-LESR)
- CLATHRIN-DEPENDENT ENDOCYTOSIS REQUIRED FOR NEURONAL CELL SURVIVAL IN PLANARIAN(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- STUDY OF GENES REQUIRED FOR BRAIN REGENERATION AND NEURONAL MAINTENANCE IN PLANARIAN(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Antiparkinsonian Drugs and Their Neuroprotective Effects
- Protective Effect of Talipexole on MPTP-Treated Planarian, a Unique Parkinsonian Worm Model
- Oxovanadium(IV)-Porphyrin Complex as a Potent Insulin-Mimetic. Treatment of Experimental Type 1 Diabetic Mice by the Complex [meso-Tetrakis(4-sulfonatophenyl)porphyrinato]oxovanadate(IV)(4-)
- Merallokinetic Study of Zinc in the Blood of Normal Rats Given Insulinomimetic Zinc(II) Complexes and Improvement of Diabetes Mellitus in Type 2 Diabetic GK Rats by their Oral Administration
- A New Determination Method of Nitric Oxide (NO) with a NO-selective Electrode Demonstrated by the Kinetic Analysis of NO Generation and Decomposition
- Novel screening method for ultraviolet protection : Combination of a human skin-equivalent model and 8-hydroxy-2'-deoxyguanosine
- Chemical Structure-Dependent Differential Effects of Flavonoids on the Catalase Activity as Evaluated by a Chemiluminescent Method(Analytical Biochemistry)
- P-12 NATURAL PRODUCTS SYNTHESIS FEATURED BY SEQUENCIAL OLEFIN METATHESIS
- A New Type of Orally Active Insulin-Mimetic Vanadyl Complex: Bis(l-oxy-2-pyridinethiolato)oxovanalum(IV)with VO(S_2O_2) Coordination Mode
- Evidence for Singlet Oxygen Involvement in Rat and Human Cytochrome P450-dependent Substrate Oxidations
- Fatigue Properties of TiNi Shape Memory Alloy
- A New Type of Orally Active Insulin-mimetic Vanadyl-porphyrin Complex : meso-Tetrakis({4-sulfonatophenyl}porphyrinato)oxovanadium(IV)
- Determination of trace element concentrations in ancient rices (red and black rices) and a present-day rice (Koshihikari) : Relationship among the trace element concentrations, species, harvest site and rice parts
- Construction of Nonadjacent Stereocenters Containing a Trifluoromethylated Carbon by Organocatalyzed Michael Addition of β-Ketoesters to 2-(Trifluoromethyl)acrylate
- Essential Role of Singlet Oxygen Species in Cytochrome P450-dependent Substrate Oxygenation by Rat Liver Microsomes
- Possible Involvement of Singlet Oxygen Species as Multiple Oxidants in P450 Catalytic Reactions
- Direct Reaction between Shikonin and Thiols Induces Apoptosis in HL60 Cells
- Neuroprotective effect of a new DJ-1-binding compound against neurodegeneration in Parkinson's disease and stroke model rats
- Total Syntheses of (+)-1893B and Its Three Diastereomers and Evaluation of Their Biological Activities
- Development of a Novel High-Throughput Analytical Methodology, Multiple Injection Method, for Quantitative Analysis in Drug Metabolism and Pharmacokinetic Studies Using Liquid Chromatography with Tandem Mass Spectrometry
- Suppressive effects of zinc compounds against UVA-induced reactive oxygen species (ROS) in the skin of hairless mice
- Cellular Mechanism of Zinc-Hinokitiol Complexes in Diabetes Mellitus
- The physiological effects of the undercurrent water from Mt. Fuji on type 2 diabetic KK-A^y mice
- Protection Against Dopaminergic Neurodegeneration in Parkinson’s Disease–Model Animals by a Modulator of the Oxidized Form of DJ-1, a Wild-type of Familial Parkinson’s Disease–Linked PARK7
- Rakicidin A effectively induces apoptosis in hypoxia adapted Bcr-Abl positive leukemic cells
- Protective effect of metallogluconates against UVA-induced cutaneous lesion in HR-1 hairless mice
- Oral Administration of Bis(aspirinato)zinc(II) Complex Ameliorates Hyperglycemia and Metabolic Syndrome-Like Disorders in Spontaneously Diabetic KK-Ay Mice: Structure–Activity Relationship on Zinc–Salicylate Complexes
- Specific Binding of Vanadyl Ion (VO^) with Thiolate of the Cysteine-34 Residue in Serum Albumin, Demonstrated by CD Spectroscopy and Kinetic Property
- Molecular Approaches to the Treatment, Prophylaxis, and Diagnosis of Alzheimer's Disease: Tangle Formation, Amyloid-β, and Microglia in Alzheimer's Disease
- Protection Against Dopaminergic Neurodegeneration in Parkinson's Disease-Model Animals by a Modulator of the Oxidized Form of DJ-1, a Wild-type of Familial Parkinson's Disease-Linked PARK7
- In Vitro Study on the Transport of Zinc across Intestinal Epithelial Cells Using Caco-2 Monolayers and Isolated Rat Intestinal Membranes
- Pharmacological and Pharmacokinetic Studies of Anti-diabetic Tropolonato–Zn(II) Complexes with Zn(S2O2) Coordination Mode
- Effect of Selective Serotonin Reuptake Inhibitors via 5-HT_ Receptors on L-DOPA-Induced Rotational Behavior in a Hemiparkinsonian Rat Model
- Detoxification of 6-Hydroxydopamine-Induced Dopaminergic Neurodegeneration by 5,5-Dimethyl-1-pyrroline N-Oxide, a Radical Trapper, in Hemiparkinsonian Rats
- Effect of Selective Serotonin Reuptake Inhibitors via 5-HT1A Receptors on L-DOPA-Induced Rotational Behavior in a Hemiparkinsonian Rat Model
- Development of a Highly Sensitive Methodology for Quantitative Determination of Fexofenadine in a Microdose Study by Multiple Injection Method Using Ultra-High Performance Liquid Chromatography with Tandem Mass Spectrometry
- Effect of a peroxynitrite scavenger, a manganese-porphyrin compound on airway remodeling in a murine asthma
- Testicular Mineralization in KK-Ay Mice Treated with an Oxovanadium Complex