FORMATION OF ALIPHATIC ALDEHYDES IN RAT PLASMA AND LIVER DUE TO VITAMIN E DEFICIENCY(Communications to the Editor)

概要

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Three weeks old Wistar male rats were fed a Vitamin E (E) deficient or supplemented (2-ambo-α-tocopheryl acetate 20 mg/kg) diet for 5 or 8 weeks and the contents of aliphatic aldehydes in the plasma and liver were analyzed using HPLC with a fluorometric detector. Among 8 aldehydes examined, the levels of hexanal and (E)-4-hydroxy-2-nonenal (4-HN) were significantly higher in E-deficient rats than in E-supplemented rats. The liver contents of hexanal and 4-HN were positively correlated with thiobarbituric acid-reacting substances (TBARS) (γ = 0.97, 0.93). The elevated aldehyde levels in E-deficient rats were due not only to the increased formation of TBARS but also to the decrease of the liver aldehyde dehydrogenase (EC 1.2.1.3, ALDH). There was also a good negative correlation between the content of hexanal and the activity of ALDH in the liver (γ = -0.94). The accumulation of 4-HN was due, at least in part, to its limited use as a substrate for ALDH.
社団法人日本薬学会の論文
1986-12-25