紅茶の水溶性高分子画分中の抗酸化成分に関する研究

概要

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Chemical structures of antioxidative components in an aqueous non-dialyzable fraction prepared from black tea infusion (BTND) and its antioxidative activities in mice were investigated. The yield of S-2, which was a main antioxidative fraction, from the supernatant of BTND treated with 50% ethanol was 50.7%, and its phenol and sugar contents were 0.83 mg/mg and 0.12 mg/mg. These values were almost the same as those of BTND-E1 and BTND5-11 which were the fractions exhibiting the antitumor promoting activity. The chromatogram of S-2 by gel-filtration with Toyopearl HW-50 column was also similar to that of BTN5-11. The FT-IR spectra of S-2 and BTN5-11 were similar to those of tea catechins and theaflavins, and they indicated the presence of phenolic hydroxyl and esterified carboxyl groups, carbonyl groups in cycloalkane and cyclic ether structure. Those results and some data in our previous reports suggest that BTND would be a complex mixture of mixed-type and/or hydrolysable type tannins with galloyl catechin, flavonol, and sugar moieties. Oral administration of BTND at doses of 100 and 200 mg/kg body weight tended to increase the antioxidative activities in mouse plasma after 1 hr. Antioxidative activity of BTND could contribute to its antitumor promoting activity.
2009-01-31