<論文>紅茶の水溶性高分子成分の抗酸化活性

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Antioxidative activity of the aqueous non-dialyzable fraction prepared from black tea infusion was investigated. Antioxidative activities of green and black tea infusions were higher than those of oolong and Pu-erh tea. Black tea infusion was fractionated by extracting with chloroform, ethyl acetate, and n-butanol in this order and the residual aqueous layer was dialyzed. High antioxidative activities were determined in ethyl acetate extract, n-butanol extract, and aqueous non-dialysate. The aqueous non-dialysate from black tea infusion was treated with 50% ethanol and the supernatant and pellet were prepared. Almost of the components was in the supernatant (76%). Antioxidative activity of this fraction was the same as compared to that of the aqueous non-dialysate from black tea infusion. The supernatant fraction was separated through Sephadex LH-20 column chromatography and three brown polyphenols, S-1, S-2, and S-3, were obtained. The yields of S-2 and S-3 were 51% and 18%, respectively. The yield and antioxidative activity of S-l was lower than those of S-2 and S-3. Then S-2 and S-3 were thought to be the main antioxidative components in the aqueous non-dialysate from black tea infusion.
1999-01-29