生体試料の前処理法として水系ゲルろ過カラムを接続したクロマトグラフィー

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概要

• 論文の詳細を見る

• A simplified coupled column technique for the simultaneous determination of cilostamide and its metabolites in biological fluid by high performance liquid chromatography (HPLC) was developed. In this method, the plasma and urine containing an intact drug and its metabolites are injected into HPLC system directly. The effluent from the primary exclusion column is passed continuously through a four-port valve to waste. When monitoring of the elution of the biopolymers by the refractive index (RI) detector is completed, the valve diverts the metabolites and intact drug concentrated on the hydrophilic gel permeation column to the secondary column for separation. Their separation is achieved by using a gradient elution to elute the sample from the secondary column, and their detection is performed by a ultraviolet (UV) detector and a fluorescence (FL) detector. The detection limit is about 3 ng with the injection volume used in this method, and the coefficient of variation is less than 1% in retention time and less than 3% in the peak height.

• 公益社団法人日本薬学会の論文
• 1983-11-25

著者

• 中川 量之

  2nd Tokushima Institute Of New Drugs Research Otsuka Pharmaceutical Co. Ltd.

• 中川 量之

  大塚製薬株式会社有機化学研究所

• 中川 量之

  大塚製薬株式会社徳島研究所

• 久保 正則

  大塚製薬株式会社徳島研究所

• 清水 剛文

  Tokushima Research Institute, Otsuka Pharmaceutical Co., Ltd.,

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