Syntheses of Nitro-Substituted Aryl Diazirines. An Entry to Chromogenic Carbene Precursors for Photoaffinity Labeling
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概要
- 論文の詳細を見る
3-Phenyl-3-trifluoromethyl-diazirine derivatives with nitro and alkoxy substituents on their aromatic ring have been synthesized as carbene precursors for chromogenic detection of photolabeled products. Photolysis of the diazirines in methanol or cyclohexane gave intermolecular O-H or C-H insertion products, respectively. Spectroscopic properties of the photoproducts are such that detection of photolabeled products may be performed in a spectral region where the absorption due to most biological macromolecules is negligible. A carbon-14 analog of the diazirine was also prepared for microscale detection of labeled products. These nitro-substituted aryl diazirines should be applicable to a wide range of photoaffinity labeling studies, from tracer experiments to preparative isolation of labeled products by HPLC with spectrophotometric detection.
- 公益社団法人日本薬学会の論文
- 1994-04-15
著者
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橋本 誠
Faculty Of Pharmaceutical Sciences Hokkaido University
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中山 仁
Faculty Of Pharmaceutical Sciences Kumamoto University
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金岡 祐一
Toyama Women's College
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金岡 裕一
Faculty Of Pharmaceutical Sciences Hokkaido University
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畑中 保丸
Research Institute for Wakan-Yaku, Toyama Medical and Pharmaceutical University
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畑中 保丸
富山医科薬学大学和漢薬研究所
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中山 仁
Faculty Of Pharmaceutical Sciences Hokkaido University
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中山 仁
北大薬
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Hashimoto Makoto
Div. Of Applied Sci. Graduate School Of Agriculture Hokkaido Univ.
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Hashimoto Makoto
Department Of Agricultural And Life Science Obihiro University Of Agriculture And Veterinary Medicin
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